Difference between revisions of "Transformation of 15-Keto-PGE2 to 13,14-Dihydro-15-Keto-PGE2"

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! Reference
 
! Reference
 
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|-
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| 0.0006
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| 0.01121 ± 0.00014
 
| mM
 
| mM
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| Unkown
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| Human
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| Follows a ping-pong mechanism by binding with NADP+, transforming it to NADPH and releasing it before binding 5S-HETE.
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|Method: In vitro
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| <ref name="Steinhilber"> [https://books.google.co.uk/books?id=mEYWDAAAQBAJ&pg=PA188&lpg=PA188&dq=5-HEDH+Km&source=bl&ots=dwhpDJqBhn&sig=jgK1WqNLlylOaNedgGdwNBQN7TM&hl=en&sa=X&ved=0ahUKEwiw_pCykbfPAhVDD8AKHZu2BnwQ6AEIHDAA#v=onepage&q=5-HEDH%20Km&f=false Lipoxygenases in Inflammation - edited by Dieter Steinhilber]</ref>
 
−
|-
 
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| 0.00067
 
−
| mM
 
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| Human Cell lines
 
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| Follows a ping-pong mechanism by binding with NADP+ (Km 139 nM), transforming it to NADPH (inhibited by NADPH (Ki 224 nM)) and releasing it before binding 5S-HETE.
 
−
Method: In vitro
 
 
Organism: Human
 
Organism: Human
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Expression vector: U937 and HL-60 cell lines
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Expression vector: E.coli
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Enzyme:5-HEDH
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Enzyme: PTGR2
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pH: 7.4
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pH: 7.5
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Temperature:  37 ◦C  
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Temperature:  37 ◦C
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| <ref name="ERLEMANN2007"> [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828885/pdf/bj4030157.pdf Karl-Rudolf ERLEMANN, Regulation of 5-hydroxyeicosanoid dehydrogenase activity in
+
Substrate: 15-Keto-PGE2
−
monocytic cells, Biochem. J. (2007) 403, 157–165]</ref>  
+
 
 +
"For determining the KM and Vmax values for
 +
NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different
 +
concentrations of NADPH (0–60 mM)."
 +
 
 +
| <ref name="Wu2008"> [https://www.ncbi.nlm.nih.gov/pubmed/19000823 Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2, Structure. 2008 Nov 12;16(11):1714-23. doi: 10.1016/j.str.2008.09.007.]</ref>  
 
|-
 
|-
−
| 0.000516 ± 0.00019
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|0.01587 ± 0.00171
−
| mM
+
|mM
−
| Human Cell lines
+
|Human
−
|
+
|Method: In vitro
−
Method: In vitro
 
 
Organism: Human
 
Organism: Human
−
Expression vector: U937
+
Expression vector: E.coli
−
Enzyme:5-HEDH
+
Enzyme: e PTGR2
−
pH: 7.4
+
pH: 7.5
−
Temperature:  37 ◦C  
+
Temperature:  37 ◦C
−
| <ref name="Patel2009"> [http://jpet.aspetjournals.org/content/jpet/329/1/335.full.pdf Karl-Pranav Patel, Selectivity of 5-Hydroxyeicosanoid Dehydrogenase
+
Substrate: NADPH
−
and Its Inhibition by 5-Hydroxy-Long-Chain Fatty Acids, Journal of Pharmacology and Experimental Therapeutics April 2009, 329 (1) 335-341; ]</ref>
+
 
−
|}
+
"For determining the KM and Vmax values for
 +
NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different
 +
concentrations of NADPH (0–60 mM)."
  
−
{|class="wikitable"  
+
| <ref name="Wu2008"> [https://www.ncbi.nlm.nih.gov/pubmed/19000823 Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2, Structure. 2008 Nov 12;16(11):1714-23. doi: 10.1016/j.str.2008.09.007.]</ref>
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|+  style="text-align: left;" | Michaelis-Menten Constants
 
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! Value
 
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! Units
 
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! Species
 
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! Notes
 
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! Reference
 
 
|-
 
|-
−
| X
+
 
−
| Y
 
−
| Z
 
−
| A
 
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| B
 
 
|}
 
|}
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 +
  
 
{|class="wikitable"  
 
{|class="wikitable"  

Revision as of 10:27, 13 March 2017

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Reaction

Chemical equation

Failed to parse (Cannot store math image on filesystem.): 15-Keto-PGE2 \rightleftharpoons 13,14-Dihydro-15-Keto-PGE2

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
0.01121 ± 0.00014 mM Human Method: In vitro

Organism: Human Expression vector: E.coli Enzyme: PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: 15-Keto-PGE2

"For determining the KM and Vmax values for NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different concentrations of NADPH (0–60 mM)."

[1]
0.01587 ± 0.00171 mM Human Method: In vitro

Organism: Human Expression vector: E.coli Enzyme: e PTGR2 pH: 7.5 Temperature: 37 ◦C Substrate: NADPH

"For determining the KM and Vmax values for NADPH, 15-keto-PGE2 at a final concentration of 200 mM was used with different concentrations of NADPH (0–60 mM)."

[1]


Enzyme Turnover Number
Value Units Species Notes Reference
X Y Z A B
5-HEDH Abundance
Value Units Species Notes Reference
X Y Z A B
Gibbs Free energy
Value Units Species Notes Reference
X Y Z A B


Half Life
Value Units Species Notes Reference
9.9 +/-0.2 minutes Dog In vivo

Temperature:37 pH:7

[2]
  1. ↑ 1.0 1.1 Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2, Structure. 2008 Nov 12;16(11):1714-23. doi: 10.1016/j.str.2008.09.007.
  2. ↑ W. Bothwell, "A radioimmunoassay for the unstable pulmonary metabolites of prostaglandin E1 and E2: an indirect index of their in vivo disposition and pharmacokinetics" Journal of Pharmacology and Experimental Therapeutics February 1982, 220 (2) 229-235
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