Difference between revisions of "Transformation of 15-Keto-PGE2 to 13,14-Dihydro-15-Keto-PGE2"

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== Parameters ==
 
== Parameters ==
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{|class="wikitable"
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|+  style="text-align: left;" | Michaelis-Menten Constants
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! Value
 +
! Units
 +
! Species
 +
! Notes
 +
! Reference
 +
|-
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| 0.0006
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| mM
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| Unkown
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| Follows a ping-pong mechanism by binding with NADP+, transforming it to NADPH and releasing it before binding 5S-HETE.
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| <ref name="Steinhilber"> [https://books.google.co.uk/books?id=mEYWDAAAQBAJ&pg=PA188&lpg=PA188&dq=5-HEDH+Km&source=bl&ots=dwhpDJqBhn&sig=jgK1WqNLlylOaNedgGdwNBQN7TM&hl=en&sa=X&ved=0ahUKEwiw_pCykbfPAhVDD8AKHZu2BnwQ6AEIHDAA#v=onepage&q=5-HEDH%20Km&f=false Lipoxygenases in Inflammation - edited by Dieter Steinhilber]</ref>
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|-
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| 0.00067
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| mM
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| Human Cell lines
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| Follows a ping-pong mechanism by binding with NADP+ (Km 139 nM), transforming it to NADPH (inhibited by NADPH (Ki 224 nM)) and releasing it before binding 5S-HETE.
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Method: In vitro
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Organism: Human
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Expression vector: U937 and HL-60 cell lines
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Enzyme:5-HEDH
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pH: 7.4
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Temperature:  37 ◦C
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| <ref name="ERLEMANN2007"> [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828885/pdf/bj4030157.pdf Karl-Rudolf ERLEMANN, Regulation of 5-hydroxyeicosanoid dehydrogenase activity in
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monocytic cells, Biochem. J. (2007) 403, 157–165]</ref>
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|-
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| 0.000516 ± 0.00019
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| mM
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| Human Cell lines
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|
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Method: In vitro
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Organism: Human
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Expression vector: U937
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Enzyme:5-HEDH
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pH: 7.4
 +
Temperature:  37 ◦C
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| <ref name="Patel2009"> [http://jpet.aspetjournals.org/content/jpet/329/1/335.full.pdf Karl-Pranav Patel, Selectivity of 5-Hydroxyeicosanoid Dehydrogenase
 +
and Its Inhibition by 5-Hydroxy-Long-Chain Fatty Acids, Journal of Pharmacology and Experimental Therapeutics April 2009, 329 (1) 335-341; ]</ref>
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|}
 +
 +
{|class="wikitable"
 +
|+  style="text-align: left;" | Michaelis-Menten Constants
 +
! Value
 +
! Units
 +
! Species
 +
! Notes
 +
! Reference
 +
|-
 +
| X
 +
| Y
 +
| Z
 +
| A
 +
| B
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|}
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 +
{|class="wikitable"
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|+  style="text-align: left;" | Enzyme Turnover Number
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! Value
 +
! Units
 +
! Species
 +
! Notes
 +
! Reference
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|-
 +
| X
 +
| Y
 +
| Z
 +
| A
 +
| B
 +
|}
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{|class="wikitable"
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|+  style="text-align: left;" | 5-HEDH Abundance
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! Value
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! Units
 +
! Species
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! Notes
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! Reference
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|-
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| X
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| Y
 +
| Z
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| A
 +
| B
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|}
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 +
{|class="wikitable"
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|+  style="text-align: left;" | Gibbs Free energy
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! Value
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! Units
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! Species
 +
! Notes
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! Reference
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|-
 +
| X
 +
| Y
 +
| Z
 +
| A
 +
| B
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|}
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{|class="wikitable"  
 
{|class="wikitable"  

Revision as of 10:13, 13 March 2017

Return to overview


Reaction

Chemical equation

Failed to parse (Cannot store math image on filesystem.): 15-Keto-PGE2 \rightleftharpoons 13,14-Dihydro-15-Keto-PGE2

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
0.0006 mM Unkown Follows a ping-pong mechanism by binding with NADP+, transforming it to NADPH and releasing it before binding 5S-HETE. [1]
0.00067 mM Human Cell lines Follows a ping-pong mechanism by binding with NADP+ (Km 139 nM), transforming it to NADPH (inhibited by NADPH (Ki 224 nM)) and releasing it before binding 5S-HETE.

Method: In vitro Organism: Human Expression vector: U937 and HL-60 cell lines Enzyme:5-HEDH pH: 7.4 Temperature: 37 ◦C

[2]
0.000516 ± 0.00019 mM Human Cell lines

Method: In vitro Organism: Human Expression vector: U937 Enzyme:5-HEDH pH: 7.4 Temperature: 37 ◦C

[3]
Michaelis-Menten Constants
Value Units Species Notes Reference
X Y Z A B
Enzyme Turnover Number
Value Units Species Notes Reference
X Y Z A B
5-HEDH Abundance
Value Units Species Notes Reference
X Y Z A B
Gibbs Free energy
Value Units Species Notes Reference
X Y Z A B


Half Life
Value Units Species Notes Reference
9.9 +/-0.2 minutes Dog In vivo

Temperature:37 pH:7

[4]
  1. ↑ Lipoxygenases in Inflammation - edited by Dieter Steinhilber
  2. ↑ [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828885/pdf/bj4030157.pdf Karl-Rudolf ERLEMANN, Regulation of 5-hydroxyeicosanoid dehydrogenase activity in monocytic cells, Biochem. J. (2007) 403, 157–165]
  3. ↑ [http://jpet.aspetjournals.org/content/jpet/329/1/335.full.pdf Karl-Pranav Patel, Selectivity of 5-Hydroxyeicosanoid Dehydrogenase and Its Inhibition by 5-Hydroxy-Long-Chain Fatty Acids, Journal of Pharmacology and Experimental Therapeutics April 2009, 329 (1) 335-341; ]
  4. ↑ W. Bothwell, "A radioimmunoassay for the unstable pulmonary metabolites of prostaglandin E1 and E2: an indirect index of their in vivo disposition and pharmacokinetics" Journal of Pharmacology and Experimental Therapeutics February 1982, 220 (2) 229-235
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