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| Line 27: |
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| | == Parameters == | | == Parameters == |
| − | {|class="wikitable sortable"
| |
| − | |+ style="text-align: left;" | Michaelis-Menten Constants
| |
| − | |-
| |
| − | ! Value
| |
| − | ! Units
| |
| − | ! Species
| |
| − | ! Notes
| |
| − | ! Reference
| |
| − | |-
| |
| − | |0.0054
| |
| − | |mM
| |
| − | |Human Eye
| |
| − | |Vector: HEK-OATP2A1 or HEK-OATP2B1 and the respective vector controls
| |
| − | Substrate: Latanoprost, a prostaglandin F2a analogue
| |
| − | pH: 7.5
| |
| − | Temp: 37 °C
| |
| − | |<ref name="Kraft2010"> [https://www.ncbi.nlm.nih.gov/pubmed/20019365 M. Kraft ''The prostaglandin transporter OATP2A1 is expressed in human ocular tissues and transports the antiglaucoma prostanoid latanoprost.'' Invest Ophthalmol Vis Sci. 2010 May;51(5):2504-11. doi: 10.1167/iovs.09-4290. Epub 2009 Dec 17.]</ref>
| |
| − | |-
| |
| − | |0.000331 ± 0.000131
| |
| − | |mM
| |
| − | |Transfected Human embryonic kidney cells (HEK293)
| |
| − | |Substrate: PGE2
| |
| − | pH: unknown
| |
| − | Temp: 37 °C
| |
| − | |<ref name="Gose2016"> [https://www.ncbi.nlm.nih.gov/pubmed/26692285 Gose T. ''Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3.'' Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.]</ref>
| |
| − | |-
| |
| − | |0.007202 ± 0.000595
| |
| − | |mM
| |
| − | |Transfected Human embryonic kidney cells (HEK293)
| |
| − | |Substrate: PGE3
| |
| − | pH: unknown
| |
| − | Temp: 37 °C
| |
| − | |<ref name="Gose2016"> [https://www.ncbi.nlm.nih.gov/pubmed/26692285 Gose T. ''Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3.'' Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.]</ref>
| |
| − | |-
| |
| − | |0.000376 ± 3.4e-5
| |
| − | |mM
| |
| − | |PGT-expressing Madin-Darby Canine Kidney Epithelial Cells (MDCK) cells
| |
| − | |Substrate: PGH2
| |
| − | pH: unknown
| |
| − | Temp: unknown
| |
| − | |<ref name="Chi2010"> [http://www.sciencedirect.com/science/article/pii/S0006291X10005723 Chi Y. ''The prostaglandin transporter PGT transports PGH2'' Biochemical and Biophysical Research Communications, Volume 395, Issue 2, 30 April 2010, Pages 168–172]</ref>
| |
| − | |-
| |
| − | |9.40E-05
| |
| − | |mM
| |
| − | |Human
| |
| − | |Substrate: PGE2
| |
| − | Species: Human
| |
| − | Vector: HeLa Cell Line
| |
| − | pH: 7
| |
| − | Temperature: 27
| |
| − | |<ref name="Kanai1995"> [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. ''Identification and Characterization of a
| |
| − | Prostaglandin Transporter'' Science 12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]</ref>
| |
| − | |-
| |
| − | |0.000423
| |
| − | |mM
| |
| − | |Human
| |
| − | |Substrate: TXB2
| |
| − | Species: Human
| |
| − | Vector: HeLa Cell Line
| |
| − | pH: 7
| |
| − | Temperature: 27
| |
| − | |<ref name="Kanai1995"> [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. ''Identification and Characterization of a
| |
| − | Prostaglandin Transporter'' Science 12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]</ref>
| |
| − | |-
| |
| − | |0.007569
| |
| − | |mM
| |
| − | |Human
| |
| − | |Substrate: 6-Keto-PGF1a
| |
| − | Species: Human
| |
| − | Vector: HeLa Cell Line
| |
| − | pH: 7
| |
| − | Temperature: 27
| |
| − | |<ref name="Kanai1995"> [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. ''Identification and Characterization of a
| |
| − | Prostaglandin Transporter'' Science 12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]</ref>
| |
| − | |-
| |
| − | |4.87E-05
| |
| − | |mM
| |
| − | |Human
| |
| − | |Substrate: PGE2
| |
| − | Species: Human
| |
| − | Vector: HeLa Cell Line
| |
| − | pH: 7
| |
| − | Temperature: 27
| |
| − | |<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref>
| |
| − | |-
| |
| − | |5.02E-05
| |
| − | |mM
| |
| − | |Human
| |
| − | |Substrate: PGF2a
| |
| − | Species: Human
| |
| − | Vector: HeLa Cell Line
| |
| − | pH: 7
| |
| − | Temperature: 27
| |
| − | |<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref>
| |
| − | |-
| |
| − | |0.005
| |
| − | |mM
| |
| − | |Human
| |
| − | |Substrate: 15-Keto-PGE2
| |
| − | Species: Human
| |
| − | Vector: HeLa Cell Line
| |
| − | pH: 7
| |
| − | Temperature: 27
| |
| − | |<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref>
| |
| − | |-
| |
| − | |0.005
| |
| − | |mM
| |
| − | |Human
| |
| − | |Substrate: 13,14-Dihydo-15-Keto-PGE2
| |
| − | Species: Human
| |
| − | Vector: HeLa Cell Line
| |
| − | pH: 7
| |
| − | Temperature: 27
| |
| − | |<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref>
| |
| − | |-
| |
| − | |3.82E-05
| |
| − | |mM
| |
| − | |Human
| |
| − | |Substrate: PGH2
| |
| − | Species: Human
| |
| − | Vector: HeLa Cell Line
| |
| − | pH: 7
| |
| − | Temperature: 27
| |
| − | |<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref>
| |
| − | |-
| |
| − | |}
| |
| − |
| |
| − |
| |
| − | No data available on the turnover of PGT/ an organic molecule transporter therefore based upon estimates made by http://book.bionumbers.org/what-are-the-rates-of-membrane-transporters/
| |
| − | {|class="wikitable sortable"
| |
| − | |+ style="text-align: left;" | Turnover Number
| |
| − | |-
| |
| − | ! Value
| |
| − | ! Units
| |
| − | ! Transporter
| |
| − | ! Notes
| |
| − | ! Reference
| |
| − | |-
| |
| − | |3.5
| |
| − | |min-1
| |
| − | |Glucose transporter ptsI
| |
| − | |Organism: Bacteria Escherichia coli
| |
| − | Back of the envelope calculation by BioNumbers: http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=102931
| |
| − | |<ref name="Waygood1980"> [https://www.ncbi.nlm.nih.gov/pubmed/6992959?dopt=Abstract Waygood EB ''Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties.''Can J Biochem. 1980 Jan;58(1):40-8.]</ref>
| |
| − | |-
| |
| − | |0.33 - 0.83
| |
| − | |min-1
| |
| − | |Rate of transport of H+/Lactose transporter
| |
| − | |Organism: Bacteria Escherichia coli
| |
| − | Back of the envelop calculation on bionumbers http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=103159
| |
| − | |<ref name="Naftalin2007"> [https://www.ncbi.nlm.nih.gov/pubmed/17325012?dopt=Abstract Naftalin R. J. ''ELactose permease H+-lactose symporter: mechanical switch or Brownian ratchet?''Biophys J. 2007 May 15;92(10):3474-91. Epub 2007 Feb 26.]</ref>
| |
| − | |-
| |
| − | |3.28
| |
| − | |min-1
| |
| − | |Catalytic Rate of transporter HXT7
| |
| − | |Organism: Budding yeast Saccharomyces cerevisiae
| |
| − | Back of the envelope calculation http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=101737
| |
| − | |<ref name="Ye2001"> [https://www.ncbi.nlm.nih.gov/pubmed/11561293?dopt=Abstract Ye L. ''Expression and activity of the Hxt7 high-affinity hexose transporter of Saccharomyces cerevisiae''Yeast. 2001 Sep 30;18(13):1257-67.]</ref>
| |
| − | |-
| |
| − | |}
| |
| − |
| |
| − | {|class="wikitable sortable"
| |
| − | |+ style="text-align: left;" | Abundance
| |
| − | |-
| |
| − | ! Value
| |
| − | ! Units
| |
| − | ! Species
| |
| − | ! Notes
| |
| − | ! Reference
| |
| − | |-
| |
| − | |125
| |
| − | |ppm
| |
| − | |Human
| |
| − | |Vector:Lung
| |
| − | Enzyme: SLCO2A1
| |
| − | pH: 7.5
| |
| − | Temperature: 37 °C
| |
| − | |<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
| |
| − | |-
| |
| − | |17.4
| |
| − | |ppm
| |
| − | |Human
| |
| − | |Expression Vector: Esophagus
| |
| − | Enzyme: SLCO2A1
| |
| − | pH: 7.5
| |
| − | Temperature: 37 °C
| |
| − | |<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
| |
| − | |-
| |
| − | |2.82
| |
| − | |ppm
| |
| − | |Human
| |
| − | |Vector:Gut
| |
| − | Enzyme: SLCO2A1
| |
| − | pH: 7.5
| |
| − | Temperature: 37 °C
| |
| − | |<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
| |
| − | |-
| |
| − | |}
| |
| | | | |
| | == References == | | == References == |
