Difference between revisions of "Prostaglandin Transporter"
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|<ref name="Chi2010"> [http://www.sciencedirect.com/science/article/pii/S0006291X10005723 Chi Y. ''The prostaglandin transporter PGT transports PGH2'' Biochemical and Biophysical Research Communications, Volume 395, Issue 2, 30 April 2010, Pages 168–172]</ref> | |<ref name="Chi2010"> [http://www.sciencedirect.com/science/article/pii/S0006291X10005723 Chi Y. ''The prostaglandin transporter PGT transports PGH2'' Biochemical and Biophysical Research Communications, Volume 395, Issue 2, 30 April 2010, Pages 168–172]</ref> | ||
+ | |- | ||
+ | |9.40E-05 | ||
+ | |mM | ||
+ | |Human | ||
+ | |Substrate: PGE2 | ||
+ | Species: Human | ||
+ | Vector: HeLa Cell Line | ||
+ | pH: 7 | ||
+ | Temperature: 27 | ||
+ | |<ref name="Kanai1995"> [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. ''Identification and Characterization of a | ||
+ | Prostaglandin Transporter'' Science 12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]</ref> | ||
+ | |- | ||
+ | |0.000423 | ||
+ | |mM | ||
+ | |Human | ||
+ | |Substrate: TXB2 | ||
+ | Species: Human | ||
+ | Vector: HeLa Cell Line | ||
+ | pH: 7 | ||
+ | Temperature: 27 | ||
+ | |<ref name="Kanai1995"> [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. ''Identification and Characterization of a | ||
+ | Prostaglandin Transporter'' Science 12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]</ref> | ||
+ | |- | ||
+ | |0.007569 | ||
+ | |mM | ||
+ | |Human | ||
+ | |Substrate: 6-Keto-PGF1a | ||
+ | Species: Human | ||
+ | Vector: HeLa Cell Line | ||
+ | pH: 7 | ||
+ | Temperature: 27 | ||
+ | |<ref name="Kanai1995"> [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. ''Identification and Characterization of a | ||
+ | Prostaglandin Transporter'' Science 12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]</ref> | ||
+ | |- | ||
+ | |4.87E-05 | ||
+ | |mM | ||
+ | |Human | ||
+ | |Substrate: PGE2 | ||
+ | Species: Human | ||
+ | Vector: HeLa Cell Line | ||
+ | pH: 7 | ||
+ | Temperature: 27 | ||
+ | |<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref> | ||
+ | |- | ||
+ | |5.02E-05 | ||
+ | |mM | ||
+ | |Human | ||
+ | |Substrate: PGF2a | ||
+ | Species: Human | ||
+ | Vector: HeLa Cell Line | ||
+ | pH: 7 | ||
+ | Temperature: 27 | ||
+ | |<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref> | ||
+ | |- | ||
+ | |0.005 | ||
+ | |mM | ||
+ | |Human | ||
+ | |Substrate: 15-Keto-PGE2 | ||
+ | Species: Human | ||
+ | Vector: HeLa Cell Line | ||
+ | pH: 7 | ||
+ | Temperature: 27 | ||
+ | |<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref> | ||
+ | |- | ||
+ | |0.005 | ||
+ | |mM | ||
+ | |Human | ||
+ | |Substrate: 13,14-Dihydo-15-Keto-PGE2 | ||
+ | Species: Human | ||
+ | Vector: HeLa Cell Line | ||
+ | pH: 7 | ||
+ | Temperature: 27 | ||
+ | |<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref> | ||
+ | |- | ||
+ | |3.82E-05 | ||
+ | |mM | ||
+ | |Human | ||
+ | |Substrate: PGH2 | ||
+ | Species: Human | ||
+ | Vector: HeLa Cell Line | ||
+ | pH: 7 | ||
+ | Temperature: 27 | ||
+ | |<ref name="SHIGEKAZU1996"> [http://molpharm.aspetjournals.org/content/molpharm/50/4/736.full.pdf Shigekazu I. ''Structural Determinants of Substrates for the Prostaglandin Transporter PGT'' Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736]</ref> | ||
|- | |- | ||
|} | |} |
Revision as of 14:42, 2 June 2017
Contents
Reaction
Chemical equation
Rate equation
Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
0.0054 | mM | Human Eye | Vector: HEK-OATP2A1 or HEK-OATP2B1 and the respective vector controls
Substrate: Latanoprost, a prostaglandin F2a analogue pH: 7.5 Temp: 37 °C |
[1] |
0.000331 ± 0.000131 | mM | Transfected Human embryonic kidney cells (HEK293) | Substrate: PGE2
pH: unknown Temp: 37 °C |
[2] |
0.007202 ± 0.000595 | mM | Transfected Human embryonic kidney cells (HEK293) | Substrate: PGE3
pH: unknown Temp: 37 °C |
[2] |
0.000376 ± 3.4e-5 | mM | PGT-expressing Madin-Darby Canine Kidney Epithelial Cells (MDCK) cells | Substrate: PGH2
pH: unknown Temp: unknown |
[3] |
9.40E-05 | mM | Human | Substrate: PGE2
Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27 |
[4] |
0.000423 | mM | Human | Substrate: TXB2
Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27 |
[4] |
0.007569 | mM | Human | Substrate: 6-Keto-PGF1a
Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27 |
[4] |
4.87E-05 | mM | Human | Substrate: PGE2
Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27 |
[5] |
5.02E-05 | mM | Human | Substrate: PGF2a
Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27 |
[5] |
0.005 | mM | Human | Substrate: 15-Keto-PGE2
Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27 |
[5] |
0.005 | mM | Human | Substrate: 13,14-Dihydo-15-Keto-PGE2
Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27 |
[5] |
3.82E-05 | mM | Human | Substrate: PGH2
Species: Human Vector: HeLa Cell Line pH: 7 Temperature: 27 |
[5] |
No data available on the turnover of PGT/ an organic molecule transporter therefore based upon estimates made by http://book.bionumbers.org/what-are-the-rates-of-membrane-transporters/
Value | Units | Transporter | Notes | Reference |
---|---|---|---|---|
3.5 | min-1 | Glucose transporter ptsI | Organism: Bacteria Escherichia coli
Back of the envelope calculation by BioNumbers: http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=102931 |
[6] |
0.33 - 0.83 | min-1 | Rate of transport of H+/Lactose transporter | Organism: Bacteria Escherichia coli
Back of the envelop calculation on bionumbers http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=103159 |
[7] |
3.28 | min-1 | Catalytic Rate of transporter HXT7 | Organism: Budding yeast Saccharomyces cerevisiae
Back of the envelope calculation http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=101737 |
[8] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
125 | ppm | Human | Vector:Lung
Enzyme: SLCO2A1 pH: 7.5 Temperature: 37 °C |
[9] |
17.4 | ppm | Human | Expression Vector: Esophagus
Enzyme: SLCO2A1 pH: 7.5 Temperature: 37 °C |
[10] |
2.82 | ppm | Human | Vector:Gut
Enzyme: SLCO2A1 pH: 7.5 Temperature: 37 °C |
[9] |
References
- ↑ M. Kraft The prostaglandin transporter OATP2A1 is expressed in human ocular tissues and transports the antiglaucoma prostanoid latanoprost. Invest Ophthalmol Vis Sci. 2010 May;51(5):2504-11. doi: 10.1167/iovs.09-4290. Epub 2009 Dec 17.
- ↑ 2.0 2.1 Gose T. Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3. Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.
- ↑ Chi Y. The prostaglandin transporter PGT transports PGH2 Biochemical and Biophysical Research Communications, Volume 395, Issue 2, 30 April 2010, Pages 168–172
- ↑ 4.0 4.1 4.2 [http://science.sciencemag.org/content/sci/268/5212/866.full.pdf Kanai N. Identification and Characterization of a Prostaglandin Transporter Science 12 May 1995:Vol. 268, Issue 5212, pp. 866-869, DOI: 10.1126/science.7754369]
- ↑ 5.0 5.1 5.2 5.3 5.4 Shigekazu I. Structural Determinants of Substrates for the Prostaglandin Transporter PGT Molecular Pharmacology October 1996, 50 (4) 736-742; DOI: https://doi.org/10.1124/mol.50.4.736
- ↑ Waygood EB Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties.Can J Biochem. 1980 Jan;58(1):40-8.
- ↑ Naftalin R. J. ELactose permease H+-lactose symporter: mechanical switch or Brownian ratchet?Biophys J. 2007 May 15;92(10):3474-91. Epub 2007 Feb 26.
- ↑ Ye L. Expression and activity of the Hxt7 high-affinity hexose transporter of Saccharomyces cerevisiaeYeast. 2001 Sep 30;18(13):1257-67.
- ↑ 9.0 9.1 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
- ↑ M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587