Difference between revisions of "Prostaglandin Transporter"
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|mM | |mM | ||
|Human Eye | |Human Eye | ||
| − | |Substrate: Latanoprost, a prostaglandin F2a analogue | + | |Vector: HEK-OATP2A1 or HEK-OATP2B1 and the respective vector controls |
| + | Substrate: Latanoprost, a prostaglandin F2a analogue | ||
| + | pH: 7.5 | ||
| + | Temp: 37 °C | ||
|<ref name="Kraft2010"> [https://www.ncbi.nlm.nih.gov/pubmed/20019365 M. Kraft ''The prostaglandin transporter OATP2A1 is expressed in human ocular tissues and transports the antiglaucoma prostanoid latanoprost.'' Invest Ophthalmol Vis Sci. 2010 May;51(5):2504-11. doi: 10.1167/iovs.09-4290. Epub 2009 Dec 17.]</ref> | |<ref name="Kraft2010"> [https://www.ncbi.nlm.nih.gov/pubmed/20019365 M. Kraft ''The prostaglandin transporter OATP2A1 is expressed in human ocular tissues and transports the antiglaucoma prostanoid latanoprost.'' Invest Ophthalmol Vis Sci. 2010 May;51(5):2504-11. doi: 10.1167/iovs.09-4290. Epub 2009 Dec 17.]</ref> | ||
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| Line 30: | Line 33: | ||
|Transfected Human embryonic kidney cells (HEK293) | |Transfected Human embryonic kidney cells (HEK293) | ||
|Substrate: PGE2 | |Substrate: PGE2 | ||
| + | pH: unknown | ||
| + | Temp: 37 °C | ||
|<ref name="Gose2016"> [https://www.ncbi.nlm.nih.gov/pubmed/26692285 Gose T. ''Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3.'' Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.]</ref> | |<ref name="Gose2016"> [https://www.ncbi.nlm.nih.gov/pubmed/26692285 Gose T. ''Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3.'' Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.]</ref> | ||
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| Line 36: | Line 41: | ||
|Transfected Human embryonic kidney cells (HEK293) | |Transfected Human embryonic kidney cells (HEK293) | ||
|Substrate: PGE3 | |Substrate: PGE3 | ||
| + | pH: unknown | ||
| + | Temp: 37 °C | ||
|<ref name="Gose2016"> [https://www.ncbi.nlm.nih.gov/pubmed/26692285 Gose T. ''Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3.'' Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.]</ref> | |<ref name="Gose2016"> [https://www.ncbi.nlm.nih.gov/pubmed/26692285 Gose T. ''Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3.'' Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.]</ref> | ||
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| Line 42: | Line 49: | ||
|PGT-expressing Madin-Darby Canine Kidney Epithelial Cells (MDCK) cells | |PGT-expressing Madin-Darby Canine Kidney Epithelial Cells (MDCK) cells | ||
|Substrate: PGH2 | |Substrate: PGH2 | ||
| + | pH: unknown | ||
| + | Temp: unknown | ||
|<ref name="Chi2010"> [http://www.sciencedirect.com/science/article/pii/S0006291X10005723 Chi Y. ''The prostaglandin transporter PGT transports PGH2'' Biochemical and Biophysical Research Communications, Volume 395, Issue 2, 30 April 2010, Pages 168–172]</ref> | |<ref name="Chi2010"> [http://www.sciencedirect.com/science/article/pii/S0006291X10005723 Chi Y. ''The prostaglandin transporter PGT transports PGH2'' Biochemical and Biophysical Research Communications, Volume 395, Issue 2, 30 April 2010, Pages 168–172]</ref> | ||
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Revision as of 17:35, 22 May 2017
Contents
Reaction
Chemical equation
Rate equation
Parameters
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 0.0054 | mM | Human Eye | Vector: HEK-OATP2A1 or HEK-OATP2B1 and the respective vector controls
Substrate: Latanoprost, a prostaglandin F2a analogue pH: 7.5 Temp: 37 °C |
[1] |
| 0.000331 ± 0.000131 | mM | Transfected Human embryonic kidney cells (HEK293) | Substrate: PGE2
pH: unknown Temp: 37 °C |
[2] |
| 0.007202 ± 0.000595 | mM | Transfected Human embryonic kidney cells (HEK293) | Substrate: PGE3
pH: unknown Temp: 37 °C |
[2] |
| 0.000376 ± 3.4e-5 | mM | PGT-expressing Madin-Darby Canine Kidney Epithelial Cells (MDCK) cells | Substrate: PGH2
pH: unknown Temp: unknown |
[3] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| (Extensive googling can't find values - will use ABC and CIF of 10) |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 125 | ppm | Human | Vector:Lung
Enzyme: SLCO2A1 pH: 7.5 Temperature: 37 °C |
[4] |
| 17.4 | ppm | Human | Expression Vector: Esophagus
Enzyme: SLCO2A1 pH: 7.5 Temperature: 37 °C |
[5] |
| 2.82 | ppm | Human | Vector:Gut
Enzyme: SLCO2A1 pH: 7.5 Temperature: 37 °C |
[4] |
References
- ↑ M. Kraft The prostaglandin transporter OATP2A1 is expressed in human ocular tissues and transports the antiglaucoma prostanoid latanoprost. Invest Ophthalmol Vis Sci. 2010 May;51(5):2504-11. doi: 10.1167/iovs.09-4290. Epub 2009 Dec 17.
- ↑ 2.0 2.1 Gose T. Prostaglandin transporter (OATP2A1/SLCO2A1) contributes to local disposition of eicosapentaenoic acid-derived PGE3. Prostaglandins Other Lipid Mediat. 2016 Jan;122:10-7. doi: 10.1016/j.prostaglandins.2015.12.003. Epub 2015 Dec 10.
- ↑ Chi Y. The prostaglandin transporter PGT transports PGH2 Biochemical and Biophysical Research Communications, Volume 395, Issue 2, 30 April 2010, Pages 168–172
- ↑ 4.0 4.1 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
- ↑ M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
