Phosphoglycerate dehydrogenase

The enzyme Phosphoglycerate dehydrogenase (PDH) catalyzes the reaction to convert 3-phospho-D-glycerate (P3G) to phosphonooxypyruvate (PHP). The cofactors NADH and NAD⁺.

Chemical equation

$3PG \rightleftharpoons PHP$

Rate equation

Modified rate equation is used

Failed to parse (Cannot store math image on filesystem.): \frac{ serA*KcatA*\frac{[3PG]}{KA_{3PG}}}{\frac{1 + \frac{[3PG]}{KA_{3PG}} + \frac{[PHP]}{KA_{PHP}}} {1+ \frac{[SER]}{KiA_{SER}}}} Failed to parse (Cannot store math image on filesystem.): \frac{\left( \frac{serA*KcatA*\frac{[3PG]}{KA_{3PG}}} {} \right)}{}

Parameters

Parameter	Value	Units	Organism	Remarks
$serA$	1.15	mM	Escherichia coli
$KcatA$	0.55	1/s
$Ka_{3PG}$	1.2	mM
$Ka_{PHP}$	0.0032	mM
$KiA_{SER}$	0.0038	mM

Parameters with uncertainty

• serA is the enzyme concentration collected from Turnaev et. al. which is considered to be fixed.
• Data reported in Zhao et. al. mentioned that the Standard Errors of parameters were less than 10% in their data. For the sake of completeness we consider the maximum Standard Error of 10% for all the data. Considering this the Standard Error for $KcatA$ would be $0.55 \pm 0.055$. Considering three samples for the data the standard deviation for this parameter would be 0.09 (Std. Dev = Std. Error $\times \sqrt{N}$).
• For $Ka_{3PG}$ it would be $1.2 \pm 0.2$, for $Ka_{PHP}$ it is $0.0032 \pm 0.00055$ and for $KiA_{SER}$ it is $0.0038 \pm 0.00065$

Parameter	Value	Units	Organism	Remarks
$serA$	1.15	mM	Escherichia coli
$KcatA$	$0.55 \pm 0.09$	1/s
$Ka_{3PG}$	$1.2 \pm 0.2$	mM
$Ka_{PHP}$	$0.0032 \pm 0.00055$	mM
$KiA_{SER}$	$0.0038 \pm 0.00065$	mM