Difference between revisions of "Nucleosid diphosphate kinase"
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==Parameter values== | ==Parameter values== | ||
+ | * A mean body weight is defined as 75Kg in Konig et. al <ref name="Konig_2012"></ref>. The <math>V_{max}</math> is calculated by multiplying the reported value with body weight. | ||
+ | |||
{|class="wikitable" | {|class="wikitable" | ||
! Parameter | ! Parameter | ||
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|- | |- | ||
|<math>V_{max}</math> | |<math>V_{max}</math> | ||
− | |<math> | + | |<math>131.25</math> <ref name = "villar_1960"> Villar-Palasi C & Larner J (1960). ''Levels of activity of the enzymes of the glycogen cycle in rat tissues''. Arch Biochem Biophys 86, 270–273.</ref> |
|<math>min^{-1}</math> | |<math>min^{-1}</math> | ||
− | | | + | |Human Liver |
|rowspan="6"| | |rowspan="6"| | ||
|- | |- | ||
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|<math> 1.33 </math><ref name="Walter_1980">Walter P and Blobel G. (1980), ''Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum'', Proc. Natl. Acad. Sci. 77:7112-7116</ref> | |<math> 1.33 </math><ref name="Walter_1980">Walter P and Blobel G. (1980), ''Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum'', Proc. Natl. Acad. Sci. 77:7112-7116</ref> | ||
|mM | |mM | ||
+ | |Rat liver | ||
|- | |- | ||
|<math>K_{m}^{ADP}</math> | |<math>K_{m}^{ADP}</math> | ||
|<math>0.042</math><ref name="Walter_1980"></ref> | |<math>0.042</math><ref name="Walter_1980"></ref> | ||
|mM | |mM | ||
+ | |Rat liver | ||
|- | |- | ||
|<math>K_{m}^{UTP}</math> | |<math>K_{m}^{UTP}</math> | ||
|<math>16</math><ref name="Fukuchi_1994"> Fukuchi, T., Shimada, N., Hanai, N., Ishikawa, N., Watanabe, K., and Kimura, N. (1994) ''Recombinant rat nucleoside diphosphate kinase isoforms (<math>\alpha</math> and <math>\beta</math>): purification, properties and application to immunological detection of native isoforms in rat tissues. Biochim. Biophys. Acta 1205, 113–122</ref> | |<math>16</math><ref name="Fukuchi_1994"> Fukuchi, T., Shimada, N., Hanai, N., Ishikawa, N., Watanabe, K., and Kimura, N. (1994) ''Recombinant rat nucleoside diphosphate kinase isoforms (<math>\alpha</math> and <math>\beta</math>): purification, properties and application to immunological detection of native isoforms in rat tissues. Biochim. Biophys. Acta 1205, 113–122</ref> | ||
|mM | |mM | ||
+ | |Rat tissue | ||
|- | |- | ||
|<math>K_{m}^{UDP}</math> | |<math>K_{m}^{UDP}</math> | ||
|<math>0.19</math><ref name="Walter_1980"></ref> | |<math>0.19</math><ref name="Walter_1980"></ref> | ||
|mM | |mM | ||
+ | |Rat liver | ||
|- | |- | ||
|<math>K_{eq}</math> | |<math>K_{eq}</math> | ||
|<math>1</math> | |<math>1</math> | ||
|Dimensionless | |Dimensionless | ||
+ | | | ||
|} | |} | ||
Revision as of 11:50, 13 May 2014
Nucleoside-diphosphate kinases are enzymes that catalyze the exchange of phosphate groups between different nucleotides. The overall effect of NDKs is to transfer a phosphate group from a nucleoside triphosphate to a nucleoside diphosphate. Starting with ATP and UDP, the activity of NDK produces ADP and UTP.
Contents
Chemical equation
Rate equation
Random order Bi-Bi rate law is used from [1]
Parameter values
- A mean body weight is defined as 75Kg in Konig et. al [1]. The is calculated by multiplying the reported value with body weight.
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
[2] | Human Liver | |||
[3] | mM | Rat liver | ||
[3] | mM | Rat liver | ||
[4] | mM | Rat tissue | ||
[3] | mM | Rat liver | ||
Dimensionless |
Parameters with uncertainty
- The value of is reported to be of . The Std. Dev. for was considered to be of its mean value. Same error percentage is considered for .
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
[2] | Recombinant, human muscle | |||
mM | ||||
mM | ||||
mM | ||||
mM | ||||
Dimensionless |
References
- ↑ 1.0 1.1 M. König, S. Bulik, H.G. Holzhütter (2012), Quantifying the contribution of the liver to glucose homeostasis: a detailed kinetic model of human hepatic glucose metabolism, PLoS Comput. Biol., 8 (6), p. e1002577
- ↑ 2.0 2.1 Villar-Palasi C & Larner J (1960). Levels of activity of the enzymes of the glycogen cycle in rat tissues. Arch Biochem Biophys 86, 270–273.
- ↑ 3.0 3.1 3.2 Walter P and Blobel G. (1980), Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum, Proc. Natl. Acad. Sci. 77:7112-7116
- ↑ Fukuchi, T., Shimada, N., Hanai, N., Ishikawa, N., Watanabe, K., and Kimura, N. (1994) Recombinant rat nucleoside diphosphate kinase isoforms ( and ): purification, properties and application to immunological detection of native isoforms in rat tissues. Biochim. Biophys. Acta 1205, 113–122