Difference between revisions of "Monocarboxylate (Lactate) transporter"
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Reversible Michaelis-Menten rate law is used | Reversible Michaelis-Menten rate law is used | ||
− | <center><math> \frac{ | + | <center><math> \frac{V_{mf} \times \frac{[Lactate_{in}]}{Km_{Lactate_{in}}} - Vmr \times \frac{[Lactate_{out}]}{Km_{Lactate_{out}}}}{1 + \frac{[Lactate_{in}]}{Km_{Lactate_{in}}} + \frac{[Lactate_{out}]}{Km_{Lactate_{out}}}} </math></center> |
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+ | Modified rate law to take Thermodynamic constraint into consideration | ||
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+ | <center><math> \frac{V_{mf} \frac{[Lactate_{in}]}{Km_{Lactate_{in}}} \left(1 - \frac{[Lactate_{out}]}{K_{eq}[Lactate_{in}] } \right)}{1 + \frac{[Lactate_{in}]}{Km_{Lactate_{in}}} + \frac{[Lactate_{out}]}{Km_{Lactate_{out}}}} </math></center> | ||
==Parameter values== | ==Parameter values== |
Revision as of 11:34, 20 June 2014
Monocarboxylate transporters,[1] or MCTs, constitute a family of proton-linked plasma membrane transporters that carry molecules having one carboxylate group (monocarboxylates), such as lactate and pyruvate.
Contents
Chemical equation
Rate equation
Reversible Michaelis-Menten rate law is used
Modified rate law to take Thermodynamic constraint into consideration
Parameter values
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
2.5 [2] | Human tumor cells | |||
2.4 [3] | Rat liver cells | |||
4.5 [3] | mM | Rat liver cell | ||
4.54 [2] | mM | Human tumor cells |
Parameters with uncertainty
- Among different isoforms the MCT1 in Tumour cells are considered in this model. The is defined as and [2] with 6 experiments.
- In the legend of Table-1 of [3] it states Km and Vmax values for monocarboxylate transport into mouse Ehrlich Lettre ́ tumor cells were taken in to consideration. Therefore we consider these values to be for and . Here the values are given in with 7 experiments. Calculating Std. Dev. from S.E gives, for and for .
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
Human tumor cells | ||||
Human Tumor cell | ||||
mM | Human tumour cell | |||
mM | Human tumor cells |
References
- ↑ Halestrap AP, Meredith D (2004). The SLC16 gene family-from monocarboxylate transporters (MCTs) to aromatic amino acid transporters and beyond. Pflugers Arch. 447 (5): 619–28 (doi)
- ↑ 2.0 2.1 2.2 Manning Fox JE et. al. (2000). Characterisation of human monocarboxylate transporter 4 substantiates its role in lactic acid efflux from skeletal muscle. J Physiol 529(Pt2):285-293
- ↑ 3.0 3.1 3.2 Jackson VN, Halestrap AP (1996) The kinetics, substrate, and inhibitor specificity of the monocarboxylate (lactate) transporter of rat liver cells determined using the fluorescent intracellular pH indicator, 2’,7’-bis(carboxyethyl)-5(6)-carboxyfluorescein. J Biol Chem 271:861–868