Limonene Synthase: Detailed information on the kinetic values obtained from the literature
Revision as of 13:23, 14 April 2016 by Aliah.hawari (talk | contribs)
Back to terpene model MAIN page.
==Kinetic Parameters==
Parameter | Value | Units | Description | References |
---|---|---|---|---|
kcatforward | 3.9x10-5 | s-1 | Limonene synthase expressed at 37°C, 40µM GPP, Citrus sinensis, product formation 1.17x105 µM/sec
|
[1] |
[GPP] | 40 | µM | Limonene synthase expressed at 37°C, 40µM GPP, Citrus sinensis, product formation 1.17x105 µM/sec | [1] |
[GPP] | 10 | µM | Limonene synthase expressed at 37°C, 40µM GPP, Citrus sinensis, product formation 1.17x105 µM/sec, [E]: 0.3 µM
|
[1] |
[Limonene synthase] | 0.3 | µM | Limonene synthase expressed at 37°C, 10µM GPP, Citrus sinensis, product formation 1.17x105 µM/sec, [E]: 0.3 µM | [1] |
[LimSynth] | 0.07 | µM | Limonene synthase expressed at 20°C, 200µM GPP, Citrus sinensis | [2] |
KmGPP | 130 | µM | [2] | |
[LimSynth] | 0.26 | µM | Limonene synthase expressed at 37°C, 200µM GPP, Citrus sinensis, pH 7.5 | [2] |
Vmaxforward | 0.53 | µM/min | Limonene synthase expressed at 20°C, 200µM GPP, Citrus sinensis, pH7.5 | [2] |
Vmaxforward | 0.64 | µM/min | Limonene synthase expressed at 37°C, 200µM GPP | [2] |
kcatforward | 0.13 | s-1 | Limonene synthase expressed at 20°C, 200µM GPP, Citrus sinensis, pH 7.5 | [2] |
kcatforward | 0.04 | s-1 | Limonene synthase expressed at 37°C, 200µM GPP, Citrus sinensis, pH 7.5 | [2] |
Enzyme amount | 1-10 | µg | [3] | |
Enzyme amount | 10-20 | µg | [4] | |
[GPP] | 10 | µM | [3] | |
[GPP] | 2 | µM | [5] | |
[GPP] | 0.1 | µM | 0.1 -100 µM | [5] |
Km | 0.7 | µM | [5] | |
[GPP] | 100 | µM | 0.1 -100 µM | [5] |
KmGPP | 1.25 | µM | [4] | |
Specific enzyme activity | 19 | µmol/h/mg | [3] | |
Kcat | 0.3 | s-1 | [3] | |
Kcat | 0.020 | s-1 | [6] | |
[LimSynth] | 1 - 50 | µg | Amount of native or recombinant protein (E.coli) used for Limonene synthase enzyme assay | [6] |
[GPP] | 1 - 40 | µM | Substrate concentration range for enzyme assay | [6] |
Kcat | 0.024 | s-1 | Below is an excerpt from Williams 1998, but data source is from Ogura 1997, describing data from wildtype E.coli | [7] |
[GPP] | 10 | µM | [8] | |
Enzyme Amount (Possibly) | 1.25 | µg | Possible it is referring to enzyme amount used for CsTPS1 (limonene producing terpene synthase).
|
[8] |
KmGPP | 6.8 | µM | Km value for CsTPS1, pH6.5, 40°C, Cannabis sativa enzyme assay | [8] |
KmGPP | 8.6 | µM | Km value for E.coli Q53, from Williams 1998 | [6] |
KmGPP | 6.0 | µM | Km value for E.coli E57, from Williams 1998 | [6] |
KmGPP | 12.6 | µM | Km value for E.coli R58, from Williams 1998 | [6] |
KmGPP | 29.9 | µM | Km value for E.coli R59, from Williams 1998 | [6] |
KmGPP | 16 | µM | [6] | |
KmGPP | 6.7 | µM | Data from Ogura 1997, from wildtype E.coli, as described in Williams 1998. ( I will get the book!) | [7] |
Kcat | 0.082 | s-1 | Kcat value for CsTPS1, Cannabis sativa, pH6.5, 40°C, enzyme assay | [8] |
[GPP] | 25 | µM | GPP concentration used, for preprotein (?) expressed in E.coli. | [6] |
Enzyme amount | 150 | µg | Purified (-)-4S-limonene synthase amount. | [6] |
Other information
Parameter | Value | Units | Description | References |
---|---|---|---|---|
Limonene Synthase
Molecular Weight |
56 kDa | kDa | Limonene synthase purification
|
[3] |