Difference between revisions of "Limonene Synthase: Detailed information on the kinetic values obtained from the literature"
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[[file: 27_LimSynth_GPP_conc_Lucker02.png | 400px | An excerpt from Lucker 2002, Materials and Methods section]] | [[file: 27_LimSynth_GPP_conc_Lucker02.png | 400px | An excerpt from Lucker 2002, Materials and Methods section]] | ||
| + | | <ref name="Lucker2002"> </ref> | ||
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| + | | Km | ||
| + | | 0.7 | ||
| + | | µM | ||
| + | | [[file: 28_LimSynth_Km_Lucker02.png | 400px | An excerpt from Lucker 2002, Materials and Methods section]] | ||
| <ref name="Lucker2002"> </ref> | | <ref name="Lucker2002"> </ref> | ||
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Revision as of 14:40, 30 March 2016
Back to terpene model MAIN page.
| Parameter | Value | Units | Description | References |
|---|---|---|---|---|
| [LimSynth] | 0.07 | µM | Limonene synthase expressed at 20°C, 200µM GPP
|
[1] |
| KmGPP | 130 | µM | 
|
[1] |
| [LimSynth] | 0.26 | µM | Limonene synthase expressed at 37°C, 200µM GPP
|
[1] |
| Vmaxforward | 0.38 | µM/min | Limonene synthase expressed at 20°C, 200µM GPP
|
[1] |
| Vmaxforward | 0.49 | µM/min | Limonene synthase expressed at 37°C, 200µM GPP
|
[1] |
| kcatforward | 0.13 | s-1 | Limonene synthase expressed at 20°C, 200µM GPP
|
[1] |
| kcatforward | 0.04 | s-1 | Limonene synthase expressed at 37°C, 200µM GPP
|
[1] |
| Enzyme amount | 1-10 | µg | 
|
[2] |
| Enzyme amount | 10-20 | µg | 
|
[3] |
| [GPP] | 10 | µM |
|
[2] |
| [GPP] | 2 | µM | 
|
[4] |
| [GPP] | 0.1 | µM | 0.1 -100 µM
|
[4] |
| Km | 0.7 | µM | 
|
[4] |
| [GPP] | 100 | µM | 0.1 -100 µM
|
[4] |
| KmGPP | 1.25 | µM | 
|
[3] |
| Specific enzyme activity | 19 | µmol/h/mg | 
|
[2] |
| Kcat | 0.3 | s-1 |
|
[2] |
| Kcat | 0.020 | s-1 | 
|
[5] |
| Kcat | 0.024 | s-1 | Below is an excerpt from Williams 1998, but data source is from Ogura 1997, describing data from wildtype E.coli
|
[6] |
| [GPP] | 10 | µM | 
|
[7] |
| Enzyme Amount (Possibly) | 1.25 | µg | Possible it is referring to enzyme amount used for CsTPS1 (limonene producing terpene synthase).
|
[7] |
| KmGPP | 6.8 | µM | Km and Kcat value for CsTPS1 (limonene producing terpene synthase)
|
[7] |
| KmGPP | 8.6 | µM | Km value for E.coli Q53, from Williams 1998
|
[5] |
| KmGPP | 6.0 | µM | Km value for E.coli E57, from Williams 1998
|
[5] |
| KmGPP | 12.6 | µM | Km value for E.coli R58, from Williams 1998
|
[5] |
| KmGPP | 29.9 | µM | Km value for E.coli R59, from Williams 1998
|
[5] |
| KmGPP | 16 | µM |
|
[5] |
| KmGPP | 6.7 | µM | Data from Ogura 1997, from wildtype E.coli, as described in Williams 1998. ( I will get the book!)
|
[6] |
| Kcat | 0.082 | s-1 | Km and Kcat value for CsTPS1 (limonene producing terpene synthase)
|
[7] |
| [GPP] | 25 | µM | GPP concentration used, for preprotein (?) expressed in E.coli.
|
[5] |
| Enzyme amount | 150 | µg | Purified (-)-4S-limonene synthase amount.
|
[5] |
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Entova, S. 2013. "Kinetic characterization, crystallization, and photosynthetic expression of (+)-4R-limonene synthase from C. sinensis", Masters Thesis, Brandeis Univeristy
- ↑ 2.0 2.1 2.2 2.3 Alonso et. al. 1992. "Purification of 4S-Limonene Synthase, a Monoterpene Cyclase from the Glandular Trichomes of Peppermint (Mentha x piperita) and Spearmint (Mentha spicata)", The journal of Biological Chemistry, 267(11):7582-7587
- ↑ 3.0 3.1 Adam, K. et. al. 1996. "Partial purification and characterization of a monoterpene cyclase, limonene synthase, from the liverwort Ricciocarpos natans. 332(2):pp 352-356.
- ↑ 4.0 4.1 4.2 4.3 Lücker, J. et. al. 2002."Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases", Eur. J. Biochem. 269: pp3160-3171 Cite error: Invalid
<ref>tag; name "Lucker2002" defined multiple times with different content Cite error: Invalid<ref>tag; name "Lucker2002" defined multiple times with different content Cite error: Invalid<ref>tag; name "Lucker2002" defined multiple times with different content - ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 5.7 Williams, D.C. et. al. 1998. [www.ncbi.nlm.nih.gov/pubmed/9724535 "Truncation of Limonene Synthase preprotein provides a fully active 'pseudomature' form of this monoterpene cyclase and reveals the function of the amino-terminal arginine pair ",Biochemistry, 37:12213-12220] Cite error: Invalid
<ref>tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>tag; name "Williams1998" defined multiple times with different content Cite error: Invalid<ref>tag; name "Williams1998" defined multiple times with different content - ↑ 6.0 6.1 Ogura, K. & Koyama, T. 1997. "Dynamic Aspects of Natural Products Chemistry", pp 1-23 Cite error: Invalid
<ref>tag; name "Ogura1997" defined multiple times with different content - ↑ 7.0 7.1 7.2 7.3 Günnewich, N. et. al. 2006. "Functional expression and characterization of trichome-specific (-)-limonene synthase and (+)-α-pinene synthase from Cannabis sativa", Natural Product Communications, 0(0):1-10 Cite error: Invalid
<ref>tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid<ref>tag; name "Gunnewich2006" defined multiple times with different content Cite error: Invalid<ref>tag; name "Gunnewich2006" defined multiple times with different content
