Difference between revisions of "Limonene Synthase"
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! style="border: 1px solid black; padding: 5px; background: #ffdead;"| Reaction Flux (µM/s) | ! style="border: 1px solid black; padding: 5px; background: #ffdead;"| Reaction Flux (µM/s) | ||
|- style="height: 100px;" | |- style="height: 100px;" | ||
| − | | 5 || 24 || style="width: 150px;" | Escherichia coli || Possible reason for the low limonene production might due to the insufficient supply of IPP and DMAPP. || 0.0255 | + | | 5 || 24 || style="width: 150px;" | Escherichia coli || style="width: 1200px;" | Possible reason for the low limonene production might due to the insufficient supply of IPP and DMAPP <ref>Carter, Ora A. et. al.2013. [http://www.sciencedirect.com/science/article/pii/S0031942203002048 "Monoterpene biosynthesis pathway construction in Escherichia coli",Phytochemistry, 64:425–433, 2003.]</ref>. || 0.0255 |
|- style="height: 100px;" | |- style="height: 100px;" | ||
| − | | 335 || style="width: 50px;" | 48 || Escherichia coli || Engineered E.coli in which heterologous MVA pathway was installed. || 0.8537 | + | | 335 || style="width: 50px;" | 48 || Escherichia coli || Engineered E.coli in which heterologous MVA pathway was installed <ref>Alonso-Gutierez et. al. 2013. [http://www.sciencedirect.com/science/article/pii/S109671761300058X "Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production", Metabolic Engineering, 19:33-41]</ref>. || 0.8537 |
|- style="height: 100px;" | |- style="height: 100px;" | ||
| − | | 35.8 || 48 || Escherichia coli || E.coli was engineered to express GPPS, LS, DXS, and IDI. || 0.0912 | + | | 35.8 || 48 || Escherichia coli || E.coli was engineered to express GPPS, LS, DXS, and IDI <ref>Du et. al. 2014. [http://www.bioresourcesbioprocessing.com/content/1/1/10 "Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E.coli", Bioprocessing and Bioprocessing, 1:10]</ref> . || 0.0912 |
|- | |- | ||
| − | |4.87 || 48 || Escherichia coli || This was the initial titer. The study established a limonene biosynthesis pathway in E.coli using four different polycistronic operons based on 3 vectors with varied expression strength || 0.0124 | + | |4.87 || 48 || Escherichia coli || This was the initial titer. The study established a limonene biosynthesis pathway in E.coli using four different polycistronic operons based on 3 vectors with varied expression strength <ref>Du et. al. 2014. [http://www.bioresourcesbioprocessing.com/content/1/1/10 "Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E.coli", Bioprocessing and Bioprocessing, 1:10]</ref>. || 0.0124 |
|- | |- | ||
| − | | 17.4 || 48 || Escherichia coli || Using a plasmid with DXS and IDI over expressed || 0.0445 | + | | 17.4 || 48 || Escherichia coli || Using a plasmid with DXS and IDI over expressed <ref>Du et. al. 2014. [http://www.bioresourcesbioprocessing.com/content/1/1/10 "Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E.coli", Bioprocessing and Bioprocessing, 1:10]</ref>. || 0.0445 |
|- | |- | ||
| − | | 430 || 72 || Escherichia coli || || 0.7306 | + | | 430 || 72 || Escherichia coli || <ref>Alonso-Gutierez et. al. 2013. [http://www.sciencedirect.com/science/article/pii/S109671761300058X "Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production", Metabolic Engineering, 19:33-41]</ref> || 0.7306 |
|} | |} | ||
Revision as of 15:05, 19 February 2016
You can go back to main page of the kinetic model here.
Contents
What we know
Issues
reference test [1]
reference test 2[2]
reference test 3 [3]
Strategies
Reaction catalysed
Failed to parse (Cannot store math image on filesystem.): \begin{align} Geranyl diphosphate {\begin{smallmatrix}\displaystyle\longrightarrow \\ \displaystyle\longleftarrow \end{smallmatrix}} Limonene + Diphosphate \end{align}
Metabolite Background Information
Long metabolite names are abbreviated in the model for clarity and standard identification purposes.
| Metabolite | Abbreviation | Chemical Formula | Molar mass (g/mol) | ChEBI | ChEMBL | PubChem |
|---|---|---|---|---|---|---|
| Geranyldiphosphate | GPP | C10H20O7P2 | 314.209 | 17211 | 41432 | 445995 |
| Limonene | Limonene | C10H16 | 136.24 | 15384 | 449062 | 22311 or 439250 |
| Diphosphate | PP | O7P2 | 173.94 | 644102 |
Equation Rate
Strategies for estimating the kinetic parameter values
Calculating the Equilibrium Constant
Extracting Information from Limonene Production Rates
| Amount produced (mg/L) | Time (H) | Organism | Description | Reaction Flux (µM/s) |
|---|---|---|---|---|
| 5 | 24 | Escherichia coli | Possible reason for the low limonene production might due to the insufficient supply of IPP and DMAPP [4]. | 0.0255 |
| 335 | 48 | Escherichia coli | Engineered E.coli in which heterologous MVA pathway was installed [5]. | 0.8537 |
| 35.8 | 48 | Escherichia coli | E.coli was engineered to express GPPS, LS, DXS, and IDI [6] . | 0.0912 |
| 4.87 | 48 | Escherichia coli | This was the initial titer. The study established a limonene biosynthesis pathway in E.coli using four different polycistronic operons based on 3 vectors with varied expression strength [7]. | 0.0124 |
| 17.4 | 48 | Escherichia coli | Using a plasmid with DXS and IDI over expressed [8]. | 0.0445 |
| 430 | 72 | Escherichia coli | [9] | 0.7306 |
Published Kinetic Parameter Values
Simulations
References
- ↑ AHawari BMC Bioinformatics
- ↑ Carter, Ora A. et. al. "Monoterpene biosynthesis pathway construction in Escherichia coli",Phytochemistry, 64:425–433, 2003.
- ↑ Plunkett, John. "Sorrell accuses Murdoch of panic buying", The Guardian, London, 27 October 2005. Retrieved on 27 October 2005.
- ↑ Carter, Ora A. et. al.2013. "Monoterpene biosynthesis pathway construction in Escherichia coli",Phytochemistry, 64:425–433, 2003.
- ↑ Alonso-Gutierez et. al. 2013. "Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production", Metabolic Engineering, 19:33-41
- ↑ Du et. al. 2014. "Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E.coli", Bioprocessing and Bioprocessing, 1:10
- ↑ Du et. al. 2014. "Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E.coli", Bioprocessing and Bioprocessing, 1:10
- ↑ Du et. al. 2014. "Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E.coli", Bioprocessing and Bioprocessing, 1:10
- ↑ Alonso-Gutierez et. al. 2013. "Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production", Metabolic Engineering, 19:33-41
