Difference between revisions of "Limonene Synthase"
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=== Calculating the Equilibrium Constant === | === Calculating the Equilibrium Constant === | ||
− | === Extracting Information from Limonene Production Rates === | + | === Extracting Information from Limonene Production Rates === |
+ | |||
+ | {| class="wikitable" style="width: 60%; height: 200px;" | ||
+ | |- | ||
+ | ! style="border: 1px solid black; padding: 5px; background: #ffdead;"| Amount produced (mg/L) | ||
+ | ! style="border: 1px solid black; padding: 5px; background: #ffdead;"| Time (H) | ||
+ | ! style="border: 1px solid black; padding: 5px; background: #ffdead;"| Organism | ||
+ | ! style="border: 1px solid black; padding: 5px; background: #ffdead;"| Description | ||
+ | ! style="border: 1px solid black; padding: 5px; background: #ffdead;"| Reaction Flux (µM/s) | ||
+ | |- style="height: 100px;" | ||
+ | | 5 || 24 || style="width: 150px;" | Escherichia coli || Possible reason for the low limonene production might due to the insufficient supply of IPP and DMAPP. || 0.0255 | ||
+ | |- style="height: 100px;" | ||
+ | | 335 || style="width: 50px;" | 48 || Escherichia coli || Engineered E.coli in which heterologous MVA pathway was installed. || 0.8537 | ||
+ | |- style="height: 100px;" | ||
+ | | 35.8 || 48 || Escherichia coli || E.coli was engineered to express GPPS, LS, DXS, and IDI. || 0.0912 | ||
+ | |- | ||
+ | |4.87 || 48 || Escherichia coli || This was the initial titer. The study established a limonene biosynthesis pathway in E.coli using four different polycistronic operons based on 3 vectors with varied expression strength || 0.0124 | ||
+ | |- | ||
+ | | 17.4 || 48 || Escherichia coli || Using a plasmid with DXS and IDI over expressed || 0.0445 | ||
+ | |- | ||
+ | | 430 || 72 || Escherichia coli || || 0.7306 | ||
+ | |} | ||
=== Published Kinetic Parameter Values === | === Published Kinetic Parameter Values === |
Revision as of 14:56, 19 February 2016
You can go back to main page of the kinetic model here.
Contents
What we know
Issues
reference test [1]
reference test 2[2]
reference test 3 [3]
Strategies
Reaction catalysed
Failed to parse (Cannot store math image on filesystem.): \begin{align} Geranyl diphosphate {\begin{smallmatrix}\displaystyle\longrightarrow \\ \displaystyle\longleftarrow \end{smallmatrix}} Limonene + Diphosphate \end{align}
Metabolite Background Information
Long metabolite names are abbreviated in the model for clarity and standard identification purposes.
Metabolite | Abbreviation | Chemical Formula | Molar mass (g/mol) | ChEBI | ChEMBL | PubChem |
---|---|---|---|---|---|---|
Geranyldiphosphate | GPP | C10H20O7P2 | 314.209 | 17211 | 41432 | 445995 |
Limonene | Limonene | C10H16 | 136.24 | 15384 | 449062 | 22311 or 439250 |
Diphosphate | PP | O7P2 | 173.94 | 644102 |
Equation Rate
Strategies for estimating the kinetic parameter values
Calculating the Equilibrium Constant
Extracting Information from Limonene Production Rates
Amount produced (mg/L) | Time (H) | Organism | Description | Reaction Flux (µM/s) |
---|---|---|---|---|
5 | 24 | Escherichia coli | Possible reason for the low limonene production might due to the insufficient supply of IPP and DMAPP. | 0.0255 |
335 | 48 | Escherichia coli | Engineered E.coli in which heterologous MVA pathway was installed. | 0.8537 |
35.8 | 48 | Escherichia coli | E.coli was engineered to express GPPS, LS, DXS, and IDI. | 0.0912 |
4.87 | 48 | Escherichia coli | This was the initial titer. The study established a limonene biosynthesis pathway in E.coli using four different polycistronic operons based on 3 vectors with varied expression strength | 0.0124 |
17.4 | 48 | Escherichia coli | Using a plasmid with DXS and IDI over expressed | 0.0445 |
430 | 72 | Escherichia coli | 0.7306 |
Published Kinetic Parameter Values
Simulations
References
- ↑ AHawari BMC Bioinformatics
- ↑ Carter, Ora A. et. al. "Monoterpene biosynthesis pathway construction in Escherichia coli",Phytochemistry, 64:425–433, 2003.
- ↑ Plunkett, John. "Sorrell accuses Murdoch of panic buying", The Guardian, London, 27 October 2005. Retrieved on 27 October 2005.