Kinetic Model of Monoterpenoid Biosynthesis Wiki
Revision as of 13:49, 19 February 2016 by Aliah.hawari (talk | contribs) (→Strategies for Estimating Kinetic Parameter Values)
This wiki page describes the construction and simulation of a kinetic model of Monoterpenoid Biosynthesis.
Contents
Monoterpenoid Biosynthesis
Description of the model
Reversible Michaelis-Menten equation
All reactions in this model are described using reversible Michaelis-Menten equation.
Strategies for Estimating Kinetic Parameter Values
Calculating the Equilibrium Constant (Keq)
Using the equilibrium constant in the reversible Michaelis-Menten reaction reduces the need to obtain or estimate Vmaxreverse parameter value, which is often not available in literature.
Using the Haldane relationship, the equilibrium constant (Keq) can be written as:
- Failed to parse (Cannot store math image on filesystem.): {\color{Red}K_\mathrm{eq}} = \frac{Vmax_\mathrm{forward} * Km_\mathrm{product} }{Vmax_\mathrm{reverse} * Km_\mathrm{substrate}}
The reversible Michaelis-Menten rate equation can then be rewritten to contain the equilibrium constant (Keq) instead of using the ratio of the Vmax values.
- Failed to parse (Cannot store math image on filesystem.): v_\mathrm{reaction} = Vmax_\mathrm{forward} * \cfrac {\cfrac{[S]}{Km_\mathrm{substrate}} * \left ( 1 - \cfrac {[P]}{[S]* \color{red}K_\mathrm{eq}} \right )} {1 + \cfrac {[S]}{Km_\mathrm{substrate}} + \cfrac {[P]}{Km_\mathrm{product}}}
where [S] and [P] corresponds to the concentration of the substrate and product respectively.