Kinetic Model of Monoterpenoid Biosynthesis Wiki

This wiki page describes the construction and simulation of a kinetic model of Monoterpenoid Biosynthesis.

Description of the model

Limonene Synthase

Limonene-6-Hydroxylase

Reversible Michaelis-Menten equation

All reactions in this model are described using reversible Michaelis-Menten equation.

Strategies for Estimating Kinetic Parameter Values

Calculating the Equilibrium Constant (K_eq)

Using the Haldane relationship, the equilibrium constant (K_eq) can be written as:

Failed to parse (Cannot store math image on filesystem.): K_\mathrm{eq} = \frac{Vmax_\mathrm{forward} * Km_\mathrm{product} }{Vmax_\mathrm{reverse} * Km_\mathrm{substrate}}

The reversible Michaelis-Menten rate equation can then be rewritten to contain the equilibrium constant (K_eq) instead of using the ratio of the V_max values.

Failed to parse (syntax error): v_\mathrm{reaction} = V_\max * \cfrac{[S]}{Km_\mathrm{substrate}} * (\biggl 1 - \cfrac {[P]}{[S]*K_\mathrm{eq}} \biggr) = k_\mathrm{cat} [E]_0 \frac{[S]}{K_\mathrm{M} + [S]}

This reduces the need to obtain or estimate Vmax_reverse parameter value, which is often not available in literature.