Difference between revisions of "Kinetic Model of Monoterpenoid Biosynthesis Wiki"
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| + | The reversible Michaelis-Menten rate equation can then be rewritten to contain the equilibrium constant (K<sub>eq</sub>) instead of using the ratio of the V<sub>max</sub> values. | ||
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| + | :<math> | ||
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| + | v_\mathrm{reaction} = V_\max * \cfrac{[S]}{Km_\mathrm{substrate}} * (\biggl 1 - \cfrac {[P]}{[S]*K_\mathrm{eq}} \biggr) = k_\mathrm{cat} [E]_0 \frac{[S]}{K_\mathrm{M} + [S]} | ||
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| + | </math> | ||
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| + | This reduces the need to obtain or estimate Vmax<sub>reverse</sub> parameter value, which is often not available in literature. | ||
== Equilibrium Constant == | == Equilibrium Constant == | ||
== References == | == References == | ||
Revision as of 13:29, 19 February 2016
This wiki page describes the construction and simulation of a kinetic model of Monoterpenoid Biosynthesis.
Contents
Monoterpenoid Biosynthesis
Description of the model
Reversible Michaelis-Menten equation
All reactions in this model are described using reversible Michaelis-Menten equation.
Strategies for Estimating Kinetic Parameter Values
Calculating the Equilibrium Constant (Keq)
Using the Haldane relationship, the equilibrium constant (Keq) can be written as:
- Failed to parse (Cannot store math image on filesystem.): K_\mathrm{eq} = \frac{Vmax_\mathrm{forward} * Km_\mathrm{product} }{Vmax_\mathrm{reverse} * Km_\mathrm{substrate}}
The reversible Michaelis-Menten rate equation can then be rewritten to contain the equilibrium constant (Keq) instead of using the ratio of the Vmax values.
- Failed to parse (syntax error): v_\mathrm{reaction} = V_\max * \cfrac{[S]}{Km_\mathrm{substrate}} * (\biggl 1 - \cfrac {[P]}{[S]*K_\mathrm{eq}} \biggr) = k_\mathrm{cat} [E]_0 \frac{[S]}{K_\mathrm{M} + [S]}
This reduces the need to obtain or estimate Vmaxreverse parameter value, which is often not available in literature.
