List of Km values for Acetyl-CoA in HMGS reaction
Value
|
SD or SEM
|
Unit
|
Weight
|
Where did I get that?
|
Notes
|
Reference
|
43
|
NaN
|
μM
|
8
|
|
style="width: 10%;"Gene from Brassica juncea (indian mustard) and expressed in E. coli. pH 8.5, 35°C.
|
[1]
|
14
|
NaN
|
μM
|
64
|
|
pH 8.0; 30°C, gene from Saccharomyces cerevisiae
|
[2]
|
18
|
NaN
|
μM
|
64
|
|
pH 8.9; 30°C, gene from Saccharomyces cerevisiae
|
[2]
|
15.3
|
NaN
|
μM
|
64
|
|
pH 8.5, 30C, 2 cytosolic HMG-CoA synthase genes from Blatella germanica (cockroach), HMGS-1 and HMGS-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S; intronless gene, encodes for an active enzyme able to complement , eukaryotic Mev-1 cells. Measured at 30°C, initiated by adding 0.2-0.6 microgram of protein, final volume 400 microlitre, pH 7.0- 8.5.
|
[3]
|
50
|
|
μM
|
16
|
|
from chicken liver, pH 8.2, 30C, measured using spectrophometric assay.
|
[4]
|
50
|
6
|
μM
|
16
|
|
Gene from H. volcanii, expressed in H. volcanii. pH 8.0, 30C. Uncertainty measured as SEM.
|
[5]
|
270
|
NaN
|
μM
|
16
|
|
Gene from Avian liver, expressed in E. coli, pH 8.2, 30°C
|
[6]
|
290
|
22
|
μM
|
16
|
|
gene from E. coli, expressed in E. coli, pH 8.2, 30C. Uncertainty measured as SE.
|
[7]
|
294
|
NaN
|
μM
|
16
|
|
avian enzyme;wildtype and mutants; expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3), pH 8.2, temperature not mentioned.
|
[8]
|
350
|
NaN
|
μM
|
4
|
|
Gene from E. faecalis, expressed in E. coli, pH 9.8, 37C.
|
[9]
|
List of Km values for Acetoacetyl-CoA
Value
|
SD or SEM
|
Unit
|
Weight
|
Where did I get that?
|
Notes
|
Reference
|
0.35
|
NaN
|
μM
|
4
|
|
Gene from Gallus gallus, assay pH 8.2, 20°C.
|
[10]
|
0.4
|
NaN
|
μM
|
64
|
|
pH 8.0; 30°C, gene from Saccharomyces cerevisiae, kinetics measured at different pHs. Uncertainty measurement not mentioned.
|
[2]
|
3.2
|
NaN
|
μM
|
64
|
|
pH 8.9; 30°C, gene from Saccharomyces cerevisiae, kinetics measured at different pHs. Uncertainty measurement not mentioned.
|
[2]
|
0.5
|
NaN
|
μM
|
16
|
|
pH 8.5, 30C, 2 cytosolic HMG-CoA synthase genes from Blatella germanica, HMGS-1 and HMGs-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S; intronless gene, encodes for an active enzyme able to complement , eukaryotic Mev-1 cells. Measured at 30°C, initiated by adding 0.2-0.6 microgram of protein, final volume 400 microlitre, pH 7.0- 8.5.
|
[3]
|
0.85
|
naN
|
μM
|
4
|
|
Avian enzyme. wildtype and mutants; expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3), pH 8.2, temperature not mentioned.
|
[8]
|
1.19
|
0.12
|
μM
|
16
|
|
Gene from E. coli, expressed in E. coli, pH 8.2, 30°C
|
[7]
|
1.4
|
0.2
|
μM
|
16
|
|
Gene from H. volcanii, expressed in H. volcanii. pH 8.0, 30°C
|
[5]
|
10
|
NaN
|
μM
|
4
|
|
Gene from E. faecalis, expressed in E. coli, pH 9.8, 37C.
|
[9]
|