Hydroxymethyglutaryl-CoA synthase (HMGS)

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HMGS Parameter Values

List of Km values for Acetyl-CoA in HMGS reaction
Value SD or SEM Unit Weight Where did I get that? Notes Reference
43 NaN μM 8 Km values for Acetyl-CoA and Acetoacetyl-CoA style="width: 10%;"Gene from Brassica juncea (indian mustard) and expressed in E. coli. pH 8.5, 35°C. [1]
14 NaN μM 64 Km values for Acetyl-CoA and Acetoacetyl-CoA pH 8.0; 30°C, gene from Saccharomyces cerevisiae [2]
18 NaN μM 64 Km values for Acetyl-CoA and Acetoacetyl-CoA pH 8.9; 30°C, gene from Saccharomyces cerevisiae [2]
15.3 NaN μM 64 Km values for Acetyl-CoA and Acetoacetyl-CoA pH 8.5, 30C, 2 cytosolic HMG-CoA synthase genes from Blatella germanica (cockroach), HMGS-1 and HMGS-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S; intronless gene, encodes for an active enzyme able to complement , eukaryotic Mev-1 cells. Measured at 30°C, initiated by adding 0.2-0.6 microgram of protein, final volume 400 microlitre, pH 7.0- 8.5. [3]
50 μM 16 Km values for Acetyl-CoA and Acetoacetyl-CoA from chicken liver, pH 8.2, 30C, measured using spectrophometric assay. [4]
50 6 μM 16 Km values for Acetyl-CoA and Acetoacetyl-CoA Gene from H. volcanii, expressed in H. volcanii. pH 8.0, 30C. Uncertainty measured as SEM. [5]
270 NaN μM 16 Km values for Acetyl-CoA and Acetoacetyl-CoA Gene from Avian liver, expressed in E. coli, pH 8.2, 30°C [6]
290 22 μM 16 Km values for Acetyl-CoA and Acetoacetyl-CoA gene from E. coli, expressed in E. coli, pH 8.2, 30C. Uncertainty measured as SE. [7]
294 NaN μM 16 Km values for Acetyl-CoA and Acetoacetyl-CoA avian enzyme;wildtype and mutants; expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3), pH 8.2, temperature not mentioned. [8]
350 NaN μM 4 Km values for Acetyl-CoA and Acetoacetyl-CoA Gene from E. faecalis, expressed in E. coli, pH 9.8, 37C. [9]
List of Km values for Acetoacetyl-CoA
Value SD or SEM Unit Weight Where did I get that? Notes Reference
0.35 NaN μM 4 Km values for Acetyl-CoA and Acetoacetyl-CoA Gene from Gallus gallus, assay pH 8.2, 20°C. [10]
0.4 NaN μM 64 Km values for Acetyl-CoA and Acetoacetyl-CoA pH 8.0; 30°C, gene from Saccharomyces cerevisiae, kinetics measured at different pHs. Uncertainty measurement not mentioned. [2]
3.2 NaN μM 64 Km values for Acetyl-CoA and Acetoacetyl-CoA pH 8.9; 30°C, gene from Saccharomyces cerevisiae, kinetics measured at different pHs. Uncertainty measurement not mentioned. [2]
0.5 NaN μM 16 Km values for Acetyl-CoA and Acetoacetyl-CoA pH 8.5, 30C, 2 cytosolic HMG-CoA synthase genes from Blatella germanica, HMGS-1 and HMGs-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S; intronless gene, encodes for an active enzyme able to complement , eukaryotic Mev-1 cells. Measured at 30°C, initiated by adding 0.2-0.6 microgram of protein, final volume 400 microlitre, pH 7.0- 8.5. [3]
0.85 naN μM 4 Km values for Acetyl-CoA and Acetoacetyl-CoA Avian enzyme. wildtype and mutants; expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3), pH 8.2, temperature not mentioned. [8]
1.19 0.12 μM 16 Km values for Acetyl-CoA and Acetoacetyl-CoA Gene from E. coli, expressed in E. coli, pH 8.2, 30°C [7]
1.4 0.2 μM 16 Km values for Acetyl-CoA and Acetoacetyl-CoA Gene from H. volcanii, expressed in H. volcanii. pH 8.0, 30°C [5]
10 NaN μM 4 Km values for Acetyl-CoA and Acetoacetyl-CoA Gene from E. faecalis, expressed in E. coli, pH 9.8, 37C. [9]

References

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  1. ↑ Nagegowda, D. A., et al. (2004). "Brassica juncea 3-hydroxy-3-methylglutaryl (HMG)-CoA synthase 1: expression and characterization of recombinant wild-type and mutant enzymes." Biochem. J. 383: 517-527.
  2. ↑ 2.0 2.1 2.2 2.3 Middleton, B. (1972). "The kinetic mechanism of 3-Hydroxy-3-methylglutaryl-Coenzyme A Synthase from baker's yeast." Biochem. J. 126: 35-47.
  3. ↑ 3.0 3.1 Cabano, J., et al. (1997). "Catalytic properties of recombinant 3-Hydroxy-3-methylglutaryl Coenzyme A synthase-1 from Blatella Germanica." Insect Biochem. Molec. Biol. 27(6): 499-505.
  4. ↑ Reed, W. D. and M. D. Lane (1975). "Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase from chicken liver." Methods in Enzymology 35: 155-160.
  5. ↑ 5.0 5.1 VanNice, J. C., et al. (2013). "Expression in Haloferax volcanii of 3-Hydroxymethylglutaryl Coenzyme A synthase facilitates isolation and characterization of the active form of a key enzyme required for polyisoprenoid cell membrane biosynthesis in halophilic archaea." Journal of Bacteriology 195(17): 3854-3862.
  6. ↑ Misra, I., et al. (1993). "Avian 3-hydroxy-3-methylglutaryl-CoA synthase." The Journal of Biological Chemistry 288(16): 12129-12135.
  7. ↑ 7.0 7.1 Chun, K. Y., et al. (2000). "3-hydroxy-3-methylglutaryk-CoA synthase: participation of invariant acidic residues in formation of the acetyl-S-enzyme reaction intermediate." Biochemistry 39: 14670-14681.
  8. ↑ 8.0 8.1 Misra, I. and H. M. Miziorko (1996). "Evidence for the interaction of avian 3-hydroxy-3-methylglutaryl-CoA synthase histidine 264 with acetoacetyl-CoA." Biochemistry 35: 9610-9616.
  9. ↑ 9.0 9.1 Sutherlin, A., et al. (2002). "Enterococcus faecalis 3-Hydroxy-3-methylglutaryl coenzyme A synthase, an enzyme of isopentenyl diphosphate biosynthesis." Journal of Bacteriology 184(15): 4065-4070.
  10. ↑ Menahan, L. A., et al. (1981). "Interrelationships between 3-Hydroxy-3-Methylglutaryl-CoA synthase, acetoacetyl-COA and ketogenesis." Eur. J. Biochem. 119: 287-294.
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