Hydroxymethyglutaryl-CoA synthase (HMGS)

List of Km values for Acetyl-CoA in HMGS reaction

Value	SD or SEM	Unit	Weight	Where did I get that?	Notes	Reference
43	NaN	μM	8		style="width: 20%;"Gene from Brassica juncea (indian mustard) and expressed in E. coli. pH 8.5, 35°C.	Nagegowda (2014)
14	NaN	μM	64		pH 8.0; 30°C, gene from Saccharomyces cerevisiae	Middleton (1972)
18	NaN	μM	64		pH 8.9; 30°C, gene from Saccharomyces cerevisiae	Middleton (1972)
15.3	NaN	μM	64	Km values for Acetyl-CoA and Acetoacetyl-CoA	pH 8.5, 30C, 2 cytosolic HMG-CoA synthase genes from Blatella germanica (cockroach), HMGS-1 and HMGS-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S; intronless gene, encodes for an active enzyme able to complement , eukaryotic Mev-1 cells. Measured at 30°C, initiated by adding 0.2-0.6 microgram of protein, final volume 400 microlitre, pH 7.0- 8.5.	Cabana (1997)
50		μM	16		from chicken liver, pH 8.2, 30C, measured using spectrophometric assay.	Reed (1975)
50	6	μM	16		Gene from H. volcanii, expressed in H. volcanii. pH 8.0, 30C. Uncertainty measured as SEM.	VanNice (2013)
270	NaN	μM	16		Gene from Avian liver, expressed in E. coli, pH 8.2, 30°C	Misra(1993)
290	22	μM	16		gene from E. coli, expressed in E. coli, pH 8.2, 30C. Uncertainty measured as SE.	Chun (2000)
294	NaN	μM	16		avian enzyme;wildtype and mutants; expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3), pH 8.2, temperature not mentioned.	Misra (1996)
350	NaN	μM	4		Gene from E. faecalis, expressed in E. coli, pH 9.8, 37C.	Sutherlin (2002)