Difference between revisions of "Hydroxymethyglutaryl-CoA synthase (HMGS)"
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| style="width: 30%;" |[[File:Nagegowda_2014_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] | | style="width: 30%;" |[[File:Nagegowda_2014_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] | ||
| style="width: 20%;"Gene from Brassica juncea (indian mustard) and expressed in E. coli. pH 8.5, 35°C. | | style="width: 20%;"Gene from Brassica juncea (indian mustard) and expressed in E. coli. pH 8.5, 35°C. | ||
− | | Nagegowda ( | + | | <ref name=Nagegowda2014>Nagegowda, D. A., et al. (2004). "Brassica juncea 3-hydroxy-3-methylglutaryl (HMG)-CoA synthase 1: expression and characterization of recombinant wild-type and mutant enzymes." Biochem. J. 383: 517-527.</ref> |
|- | |- | ||
| 14 | | 14 | ||
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| [[File:Middleton_1972_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] | | [[File:Middleton_1972_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] | ||
| pH 8.0; 30°C, gene from Saccharomyces cerevisiae | | pH 8.0; 30°C, gene from Saccharomyces cerevisiae | ||
− | | Middleton (1972) | + | | <ref name=Middleton1972>Middleton, B. (1972). "The kinetic mechanism of 3-Hydroxy-3-methylglutaryl-Coenzyme A Synthase from baker's yeast." Biochem. J. 126: 35-47.</ref> |
|- | |- | ||
| 18 | | 18 | ||
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| [[File:Middleton_1972_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] | | [[File:Middleton_1972_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] | ||
| pH 8.9; 30°C, gene from Saccharomyces cerevisiae | | pH 8.9; 30°C, gene from Saccharomyces cerevisiae | ||
− | | | + | |<ref name=Middleton1972 /> |
|- | |- | ||
| 15.3 | | 15.3 | ||
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| [[File:Cabana_1997_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] | | [[File:Cabana_1997_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] | ||
| pH 8.5, 30C, 2 cytosolic HMG-CoA synthase genes from ''Blatella germanica'' (cockroach), HMGS-1 and HMGS-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S; intronless gene, encodes for an active enzyme able to complement , eukaryotic Mev-1 cells. Measured at 30°C, initiated by adding 0.2-0.6 microgram of protein, final volume 400 microlitre, pH 7.0- 8.5. | | pH 8.5, 30C, 2 cytosolic HMG-CoA synthase genes from ''Blatella germanica'' (cockroach), HMGS-1 and HMGS-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S; intronless gene, encodes for an active enzyme able to complement , eukaryotic Mev-1 cells. Measured at 30°C, initiated by adding 0.2-0.6 microgram of protein, final volume 400 microlitre, pH 7.0- 8.5. | ||
− | | | + | | <ref name=Cabano1997>Cabano, J., et al. (1997). "Catalytic properties of recombinant 3-Hydroxy-3-methylglutaryl Coenzyme A synthase-1 from Blatella Germanica." Insect Biochem. Molec. Biol. 27(6): 499-505.</ref> |
|- | |- | ||
| 50 | | 50 | ||
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| μM | | μM | ||
| 16 | | 16 | ||
− | | | + | | [[File:Reed_1975_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] |
| from chicken liver, pH 8.2, 30C, measured using spectrophometric assay. | | from chicken liver, pH 8.2, 30C, measured using spectrophometric assay. | ||
− | | Reed (1975) | + | | <ref name=Reed1975>Reed, W. D. and M. D. Lane (1975). "Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase from chicken liver." Methods in Enzymology 35: 155-160.</ref> |
|- | |- | ||
| 50 | | 50 | ||
Line 53: | Line 53: | ||
| μM | | μM | ||
| 16 | | 16 | ||
− | | | + | | [[File:VanNice_2013_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] |
| Gene from H. volcanii, expressed in H. volcanii. pH 8.0, 30C. Uncertainty measured as SEM. | | Gene from H. volcanii, expressed in H. volcanii. pH 8.0, 30C. Uncertainty measured as SEM. | ||
− | | VanNice (2013) | + | |<ref name=VanNice2013>VanNice, J. C., et al. (2013). "Expression in Haloferax volcanii of 3-Hydroxymethylglutaryl Coenzyme A synthase facilitates isolation and characterization of the active form of a key enzyme required for polyisoprenoid cell membrane biosynthesis in halophilic archaea." Journal of Bacteriology 195(17): 3854-3862.</ref> |
|- | |- | ||
| 270 | | 270 | ||
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| μM | | μM | ||
| 16 | | 16 | ||
− | | | + | | [[File:Misra_1993_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] |
| Gene from Avian liver, expressed in E. coli, pH 8.2, 30°C | | Gene from Avian liver, expressed in E. coli, pH 8.2, 30°C | ||
− | | Misra(1993) | + | | <ref name=Misra1993>Misra, I., et al. (1993). "Avian 3-hydroxy-3-methylglutaryl-CoA synthase." The Journal of Biological Chemistry 288(16): 12129-12135.</ref> |
|- | |- | ||
| 290 | | 290 | ||
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| μM | | μM | ||
| 16 | | 16 | ||
− | | | + | | [[File:Chun_2000_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] |
| gene from E. coli, expressed in E. coli, pH 8.2, 30C. Uncertainty measured as SE. | | gene from E. coli, expressed in E. coli, pH 8.2, 30C. Uncertainty measured as SE. | ||
− | | Chun (2000) | + | | <ref name=Chun2000>Chun, K. Y., et al. (2000). "3-hydroxy-3-methylglutaryk-CoA synthase: participation of invariant acidic residues in formation of the acetyl-S-enzyme reaction intermediate." Biochemistry 39: 14670-14681.</ref> |
|- | |- | ||
| 294 | | 294 | ||
Line 77: | Line 77: | ||
| μM | | μM | ||
| 16 | | 16 | ||
− | | | + | | [[File:Misra_1996_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] |
| avian enzyme;wildtype and mutants; expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3), pH 8.2, temperature not mentioned. | | avian enzyme;wildtype and mutants; expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3), pH 8.2, temperature not mentioned. | ||
− | | Misra (1996) | + | | <ref name=Misra1996>Misra, I. and H. M. Miziorko (1996). "Evidence for the interaction of avian 3-hydroxy-3-methylglutaryl-CoA synthase histidine 264 with acetoacetyl-CoA." Biochemistry 35: 9610-9616.</ref> |
|- | |- | ||
| 350 | | 350 | ||
Line 85: | Line 85: | ||
| μM | | μM | ||
| 4 | | 4 | ||
− | | | + | | [[File:Sutherlin_2002_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] |
| Gene from E. faecalis, expressed in E. coli, pH 9.8, 37C. | | Gene from E. faecalis, expressed in E. coli, pH 9.8, 37C. | ||
− | | Sutherlin (2002) | + | | <ref name=Sutherlin2002>Sutherlin, A., et al. (2002). "Enterococcus faecalis 3-Hydroxy-3-methylglutaryl coenzyme A synthase, an enzyme of isopentenyl diphosphate biosynthesis." Journal of Bacteriology 184(15): 4065-4070.</ref> |
|} | |} | ||
+ | |||
+ | ==References== | ||
+ | {{Reflist}} |
Revision as of 09:26, 14 June 2019
Value | SD or SEM | Unit | Weight | Where did I get that? | Notes | Reference |
---|---|---|---|---|---|---|
43 | NaN | μM | 8 | style="width: 20%;"Gene from Brassica juncea (indian mustard) and expressed in E. coli. pH 8.5, 35°C. | [1] | |
14 | NaN | μM | 64 | pH 8.0; 30°C, gene from Saccharomyces cerevisiae | [2] | |
18 | NaN | μM | 64 | pH 8.9; 30°C, gene from Saccharomyces cerevisiae | [2] | |
15.3 | NaN | μM | 64 | Km values for Acetyl-CoA and Acetoacetyl-CoA | pH 8.5, 30C, 2 cytosolic HMG-CoA synthase genes from Blatella germanica (cockroach), HMGS-1 and HMGS-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S; intronless gene, encodes for an active enzyme able to complement , eukaryotic Mev-1 cells. Measured at 30°C, initiated by adding 0.2-0.6 microgram of protein, final volume 400 microlitre, pH 7.0- 8.5. | [3] |
50 | μM | 16 | from chicken liver, pH 8.2, 30C, measured using spectrophometric assay. | [4] | ||
50 | 6 | μM | 16 | Gene from H. volcanii, expressed in H. volcanii. pH 8.0, 30C. Uncertainty measured as SEM. | [5] | |
270 | NaN | μM | 16 | Gene from Avian liver, expressed in E. coli, pH 8.2, 30°C | [6] | |
290 | 22 | μM | 16 | gene from E. coli, expressed in E. coli, pH 8.2, 30C. Uncertainty measured as SE. | [7] | |
294 | NaN | μM | 16 | avian enzyme;wildtype and mutants; expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3), pH 8.2, temperature not mentioned. | [8] | |
350 | NaN | μM | 4 | Gene from E. faecalis, expressed in E. coli, pH 9.8, 37C. | [9] |
References
Template:Reflist- ↑ Nagegowda, D. A., et al. (2004). "Brassica juncea 3-hydroxy-3-methylglutaryl (HMG)-CoA synthase 1: expression and characterization of recombinant wild-type and mutant enzymes." Biochem. J. 383: 517-527.
- ↑ 2.0 2.1 Middleton, B. (1972). "The kinetic mechanism of 3-Hydroxy-3-methylglutaryl-Coenzyme A Synthase from baker's yeast." Biochem. J. 126: 35-47.
- ↑ Cabano, J., et al. (1997). "Catalytic properties of recombinant 3-Hydroxy-3-methylglutaryl Coenzyme A synthase-1 from Blatella Germanica." Insect Biochem. Molec. Biol. 27(6): 499-505.
- ↑ Reed, W. D. and M. D. Lane (1975). "Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase from chicken liver." Methods in Enzymology 35: 155-160.
- ↑ VanNice, J. C., et al. (2013). "Expression in Haloferax volcanii of 3-Hydroxymethylglutaryl Coenzyme A synthase facilitates isolation and characterization of the active form of a key enzyme required for polyisoprenoid cell membrane biosynthesis in halophilic archaea." Journal of Bacteriology 195(17): 3854-3862.
- ↑ Misra, I., et al. (1993). "Avian 3-hydroxy-3-methylglutaryl-CoA synthase." The Journal of Biological Chemistry 288(16): 12129-12135.
- ↑ Chun, K. Y., et al. (2000). "3-hydroxy-3-methylglutaryk-CoA synthase: participation of invariant acidic residues in formation of the acetyl-S-enzyme reaction intermediate." Biochemistry 39: 14670-14681.
- ↑ Misra, I. and H. M. Miziorko (1996). "Evidence for the interaction of avian 3-hydroxy-3-methylglutaryl-CoA synthase histidine 264 with acetoacetyl-CoA." Biochemistry 35: 9610-9616.
- ↑ Sutherlin, A., et al. (2002). "Enterococcus faecalis 3-Hydroxy-3-methylglutaryl coenzyme A synthase, an enzyme of isopentenyl diphosphate biosynthesis." Journal of Bacteriology 184(15): 4065-4070.