Difference between revisions of "Hydroxymethyglutaryl-CoA synthase (HMGS)"
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| Line 29: | Line 29: | ||
| μM | | μM | ||
| 64 | | 64 | ||
| − | | | + | | [[File:Middleton_1972_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] |
| pH 8.9; 30°C, gene from Saccharomyces cerevisiae | | pH 8.9; 30°C, gene from Saccharomyces cerevisiae | ||
| Middleton (1972) | | Middleton (1972) | ||
| Line 37: | Line 37: | ||
| μM | | μM | ||
| 64 | | 64 | ||
| − | | | + | | [[File:Cabana_1997_HMGS.png|450px|Km values for Acetyl-CoA and Acetoacetyl-CoA]] |
| − | | pH 8.5, 30C, 2 cytosolic HMG-CoA synthase genes from Blatella germanica (cockroach), HMGS-1 and | + | | pH 8.5, 30C, 2 cytosolic HMG-CoA synthase genes from ''Blatella germanica'' (cockroach), HMGS-1 and HMGS-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S; intronless gene, encodes for an active enzyme able to complement , eukaryotic Mev-1 cells. Measured at 30°C, initiated by adding 0.2-0.6 microgram of protein, final volume 400 microlitre, pH 7.0- 8.5. |
| Cabana (1997) | | Cabana (1997) | ||
|- | |- | ||
Revision as of 14:58, 13 June 2019
| Value | SD or SEM | Unit | Weight | Where did I get that? | Notes | Reference |
|---|---|---|---|---|---|---|
| 43 | NaN | μM | 8 | 
|
style="width: 20%;"Gene from Brassica juncea (indian mustard) and expressed in E. coli. pH 8.5, 35°C. | Nagegowda (2014) |
| 14 | NaN | μM | 64 | 
|
pH 8.0; 30°C, gene from Saccharomyces cerevisiae | Middleton (1972) |
| 18 | NaN | μM | 64 |
|
pH 8.9; 30°C, gene from Saccharomyces cerevisiae | Middleton (1972) |
| 15.3 | NaN | μM | 64 | Km values for Acetyl-CoA and Acetoacetyl-CoA | pH 8.5, 30C, 2 cytosolic HMG-CoA synthase genes from Blatella germanica (cockroach), HMGS-1 and HMGS-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S; intronless gene, encodes for an active enzyme able to complement , eukaryotic Mev-1 cells. Measured at 30°C, initiated by adding 0.2-0.6 microgram of protein, final volume 400 microlitre, pH 7.0- 8.5. | Cabana (1997) |
| 50 | μM | 16 | from chicken liver, pH 8.2, 30C, measured using spectrophometric assay. | Reed (1975) | ||
| 50 | 6 | μM | 16 | Gene from H. volcanii, expressed in H. volcanii. pH 8.0, 30C. Uncertainty measured as SEM. | VanNice (2013) | |
| 270 | NaN | μM | 16 | Gene from Avian liver, expressed in E. coli, pH 8.2, 30°C | Misra(1993) | |
| 290 | 22 | μM | 16 | gene from E. coli, expressed in E. coli, pH 8.2, 30C. Uncertainty measured as SE. | Chun (2000) | |
| 294 | NaN | μM | 16 | avian enzyme;wildtype and mutants; expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3), pH 8.2, temperature not mentioned. | Misra (1996) | |
| 350 | NaN | μM | 4 | Gene from E. faecalis, expressed in E. coli, pH 9.8, 37C. | Sutherlin (2002) |
