HaCaT keratinocytes
Human adult low-calcium high-temperature (HaCaT) keratinocytes were purchased from CLS Cell Lines Service GmbH. This is a non-tumorigenic, spontaneously transformed, immortal human epidermal keratinocyte cell line which displays no major functional defects (Boukamp et al., 1988). The recommended cell culture medium for HaCaT keratinocytes was DMEM containing 10% FBS.