HaCaT keratinocytes
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Human adult low-calcium high-temperature (HaCaT) keratinocytes were purchased from CLS Cell Lines Service GmbH. This is a non-tumorigenic, spontaneously transformed, immortal human epidermal keratinocyte cell line which displays no major functional defects (Boukamp et al., 1988). The recommended cell culture medium for HaCaT keratinocytes was DMEM containing 10% FBS.
Methods
- Cell culture conditions and maintenance
- Cell treatments
- MTT Assay Method
- Proteomic Analysis Method
- Fatty acid analysis Method
- Eicosanoid Analysis Method