Difference between revisions of "HaCaT keratinocytes"
| Line 3: | Line 3: | ||
Human adult low-calcium high-temperature (HaCaT) keratinocytes were purchased from CLS Cell Lines Service GmbH. This is a non-tumorigenic, spontaneously transformed, immortal human epidermal keratinocyte cell line which displays no major functional defects (Boukamp et al., 1988). The recommended cell culture medium for HaCaT keratinocytes was DMEM containing 10% FBS. | Human adult low-calcium high-temperature (HaCaT) keratinocytes were purchased from CLS Cell Lines Service GmbH. This is a non-tumorigenic, spontaneously transformed, immortal human epidermal keratinocyte cell line which displays no major functional defects (Boukamp et al., 1988). The recommended cell culture medium for HaCaT keratinocytes was DMEM containing 10% FBS. | ||
| − | == Results == | + | == Experimental Methods == |
| + | * [[Materials and Methods|Materials and Methods]] | ||
| + | |||
| + | == Experimental Results == | ||
*[[HaCaT MTT Assay Results|MTT Assay Results]] | *[[HaCaT MTT Assay Results|MTT Assay Results]] | ||
*[[HaCaT Proteomic Analysis Results|Proteomic Analysis Results]] | *[[HaCaT Proteomic Analysis Results|Proteomic Analysis Results]] | ||
*[[HaCaT Fatty acid analysis Results|Fatty acid analysis Results]] | *[[HaCaT Fatty acid analysis Results|Fatty acid analysis Results]] | ||
*[[HaCaT Eicosanoid Analysis Results|Eicosanoid Analysis Results]] | *[[HaCaT Eicosanoid Analysis Results|Eicosanoid Analysis Results]] | ||
| + | |||
| + | == In Silico Experiment Results == | ||
| + | |||
| + | *[[Stimulating HaCaT keratinocytes with Calcium Ionophore |Calcium Ionophore]] | ||
| + | *[[Stimulating HaCaT keratinocytes with ATP|ATP]] | ||
| + | *[[Stimulating HaCaT keratinocytes with UVR|UVR]] | ||
| + | *[[Stimulating COX inhibited HaCaT keratinocytes with Calcium Ionophore |COX inhibitor + Calcium Ionophore]] | ||
Revision as of 14:32, 31 May 2019
Human adult low-calcium high-temperature (HaCaT) keratinocytes were purchased from CLS Cell Lines Service GmbH. This is a non-tumorigenic, spontaneously transformed, immortal human epidermal keratinocyte cell line which displays no major functional defects (Boukamp et al., 1988). The recommended cell culture medium for HaCaT keratinocytes was DMEM containing 10% FBS.
Experimental Methods
Experimental Results
- MTT Assay Results
- Proteomic Analysis Results
- Fatty acid analysis Results
- Eicosanoid Analysis Results
