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Line 13: |
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| [[Image:Row_stats_of_H_all_-_use.jpg|none|thumb|500px|Proteomic analysis of AA cascade proteins in HaCaT keratinocytes. Results shown as the raw intensities from two independent experiments of A23187 stimulated (green triangle) and unstimulated HaCaT keratinocytes (red circle), and three independent experiments of UVR stimulated HaCaT keratinocytes (blue diamond).]] | | [[Image:Row_stats_of_H_all_-_use.jpg|none|thumb|500px|Proteomic analysis of AA cascade proteins in HaCaT keratinocytes. Results shown as the raw intensities from two independent experiments of A23187 stimulated (green triangle) and unstimulated HaCaT keratinocytes (red circle), and three independent experiments of UVR stimulated HaCaT keratinocytes (blue diamond).]] |
− |
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− | {| class="wikitable"
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− | |+ style="text-align: left;" |The raw intensity of proteins detected in HaCaT keratinocytes which were unstimulated and stimulated with A23187 and UVR. Data is presented as mean ± SD for dual injections of two independent experiments of A23187 stimulated and unstimulated HaCaT keratinocytes, and three independent experiments of UVR stimulated HaCaT keratinocytes.
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− | |-
| |
− | ! style="text-align: center;" |
| |
− | ! colspan="3" style="text-align: center; font-weight:bold;" | Unstimulated
| |
− | ! colspan="3" style="text-align: center; font-weight:bold;" | A23187 (5 mM, 6h)
| |
− | ! colspan="3" style="text-align: center; font-weight:bold;" | UVR (15 mJ/cm2, 6h)
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− | |-
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center; font-weight:bold;" | Mean
| |
− | | style="text-align: center; font-weight:bold;" | SD
| |
− | | style="text-align: center; font-weight:bold;" | n
| |
− | | style="text-align: center; font-weight:bold;" | Mean
| |
− | | style="text-align: center; font-weight:bold;" | SD
| |
− | | style="text-align: center; font-weight:bold;" | n
| |
− | | style="text-align: center; font-weight:bold;" | Mean
| |
− | | style="text-align: center; font-weight:bold;" | SD
| |
− | | style="text-align: center; font-weight:bold;" | n
| |
− | |-
| |
− | | style="text-align: center;" | 12(S)-LOX
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | |-
| |
− | | style="text-align: center;" | 15-LOX-1
| |
− | | style="text-align: center;" | 1.72E+03
| |
− | | style="text-align: center;" | 0.00E+00
| |
− | | style="text-align: center;" | 1
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" | 8.28E+02
| |
− | | style="text-align: center;" | 4.66E+02
| |
− | | style="text-align: center;" | 3
| |
− | |-
| |
− | | style="text-align: center;" | 5-LOX
| |
− | | style="text-align: center;" | 1.03E+03
| |
− | | style="text-align: center;" | 0.00E+00
| |
− | | style="text-align: center;" | 1
| |
− | | style="text-align: center;" | 5.39E+02
| |
− | | style="text-align: center;" | 0.00E+00
| |
− | | style="text-align: center;" | 1
| |
− | | style="text-align: center;" | 1.57E+03
| |
− | | style="text-align: center;" | 1.83E+03
| |
− | | style="text-align: center;" | 2
| |
− | |-
| |
− | | style="text-align: center;" | 5LOX/FLAP
| |
− | | style="text-align: center;" | 1.68E+03
| |
− | | style="text-align: center;" | 8.34E+02
| |
− | | style="text-align: center;" | 3
| |
− | | style="text-align: center;" | 2.16E+03
| |
− | | style="text-align: center;" | 2.13E+03
| |
− | | style="text-align: center;" | 2
| |
− | | style="text-align: center;" | 1.31E+03
| |
− | | style="text-align: center;" | 1.30E+03
| |
− | | style="text-align: center;" | 2
| |
− | |-
| |
− | | style="text-align: center;" | LTA4H
| |
− | | style="text-align: center;" | 6.33E+04
| |
− | | style="text-align: center;" | 1.05E+04
| |
− | | style="text-align: center;" | 4
| |
− | | style="text-align: center;" | 9.33E+03
| |
− | | style="text-align: center;" | 1.43E+04
| |
− | | style="text-align: center;" | 4
| |
− | | style="text-align: center;" | 7.25E+04
| |
− | | style="text-align: center;" | 9.57E+03
| |
− | | style="text-align: center;" | 4
| |
− | |-
| |
− | | style="text-align: center;" | PHGPx
| |
− | | style="text-align: center;" | 3.42E+03
| |
− | | style="text-align: center;" | 0.00E+00
| |
− | | style="text-align: center;" | 1
| |
− | | style="text-align: center;" | 2.35E+03
| |
− | | style="text-align: center;" | 2.42E+03
| |
− | | style="text-align: center;" | 3
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | |-
| |
− | | style="text-align: center;" | COX-1
| |
− | | style="text-align: center;" | 3.05E+04
| |
− | | style="text-align: center;" | 0.00E+00
| |
− | | style="text-align: center;" | 1
| |
− | | style="text-align: center;" | 1.52E+04
| |
− | | style="text-align: center;" | 1.32E+04
| |
− | | style="text-align: center;" | 4
| |
− | | style="text-align: center;" | 3.43E+03
| |
− | | style="text-align: center;" | 4.61E+03
| |
− | | style="text-align: center;" | 6
| |
− | |-
| |
− | | style="text-align: center;" | PGFS (AKR13)
| |
− | | style="text-align: center;" | 1.24E+03
| |
− | | style="text-align: center;" | 0.00E+00
| |
− | | style="text-align: center;" | 1
| |
− | | style="text-align: center;" | 1.46E+03
| |
− | | style="text-align: center;" | 0.00E+00
| |
− | | style="text-align: center;" | 1
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | |-
| |
− | | style="text-align: center;" | H-PGDS
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" | 3.61E+02
| |
− | | style="text-align: center;" | 0.00E+00
| |
− | | style="text-align: center;" | 1
| |
− | | style="text-align: center;" | 2.71E+03
| |
− | | style="text-align: center;" | 0.00E+00
| |
− | | style="text-align: center;" | 1
| |
− | |-
| |
− | | style="text-align: center;" | cPGES
| |
− | | style="text-align: center;" | 1.97E+05
| |
− | | style="text-align: center;" | 1.05E+05
| |
− | | style="text-align: center;" | 4
| |
− | | style="text-align: center;" | 7.12E+04
| |
− | | style="text-align: center;" | 1.72E+04
| |
− | | style="text-align: center;" | 4
| |
− | | style="text-align: center;" | 2.48E+05
| |
− | | style="text-align: center;" | 9.60E+04
| |
− | | style="text-align: center;" | 6
| |
− | |-
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− | | style="text-align: center;" | mPGES-2
| |
− | | style="text-align: center;" | 1.49E+04
| |
− | | style="text-align: center;" | 5.91E+03
| |
− | | style="text-align: center;" | 4
| |
− | | style="text-align: center;" | 5.48E+04
| |
− | | style="text-align: center;" | 1.81E+04
| |
− | | style="text-align: center;" | 4
| |
− | | style="text-align: center;" | 1.57E+04
| |
− | | style="text-align: center;" | 2.77E+03
| |
− | | style="text-align: center;" | 6
| |
− | |-
| |
− | | style="text-align: center;" | 15-PGDH
| |
− | | style="text-align: center;" | 2.75E+03
| |
− | | style="text-align: center;" | 2.22E+02
| |
− | | style="text-align: center;" | 2
| |
− | | style="text-align: center;" | 1.83E+03
| |
− | | style="text-align: center;" | 1.37E+03
| |
− | | style="text-align: center;" | 3
| |
− | | style="text-align: center;" | 2.93E+03
| |
− | | style="text-align: center;" | 1.26E+03
| |
− | | style="text-align: center;" | 6
| |
− | |-
| |
− | | style="text-align: center;" | PTGR-2
| |
− | | style="text-align: center;" | 9.37E+03
| |
− | | style="text-align: center;" | 6.40E+03
| |
− | | style="text-align: center;" | 4
| |
− | | style="text-align: center;" | 6.17E+03
| |
− | | style="text-align: center;" | 4.56E+03
| |
− | | style="text-align: center;" | 3
| |
− | | style="text-align: center;" | 2.24E+03
| |
− | | style="text-align: center;" | 1.53E+03
| |
− | | style="text-align: center;" | 5
| |
− | |-
| |
− | | style="text-align: center;" | TXAS
| |
− | | style="text-align: center;" | 8.06E+03
| |
− | | style="text-align: center;" | 5.75E+03
| |
− | | style="text-align: center;" | 4
| |
− | | style="text-align: center;" | 1.31E+03
| |
− | | style="text-align: center;" | 5.89E+02
| |
− | | style="text-align: center;" | 2
| |
− | | style="text-align: center;" | 2.88E+03
| |
− | | style="text-align: center;" | 1.87E+03
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− | | style="text-align: center;" | 4
| |
− | |}
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| | | |
| == Further analysis of the proteomic dataset == | | == Further analysis of the proteomic dataset == |
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| [[Image:Proteomic_analysis_-_dave.jpg|none|thumb|500px|In-depth proteomic analysis of AA cascade proteins in HaCaT keratinocytes. Results shown are from two independent experiments of A23187 stimulated (green triangle) and unstimulated HaCaT keratinocytes (red circle), and three independent experiments of UVR stimulated HaCaT keratinocytes (blue diamond).]] | | [[Image:Proteomic_analysis_-_dave.jpg|none|thumb|500px|In-depth proteomic analysis of AA cascade proteins in HaCaT keratinocytes. Results shown are from two independent experiments of A23187 stimulated (green triangle) and unstimulated HaCaT keratinocytes (red circle), and three independent experiments of UVR stimulated HaCaT keratinocytes (blue diamond).]] |
− |
| |
− | {| class="wikitable"
| |
− | |+ style="text-align: left;" |The Log2 intensity of proteins detected in HaCaT keratinocytes which were unstimulated and stimulated with A23187 and UVR. Data is presented as mean ± SD for two independent experiments of A23187 stimulated and unstimulated HaCaT keratinocytes, and three independent experiments of UVR stimulated HaCaT keratinocytes.
| |
− | |-
| |
− | ! style="text-align: center;" |
| |
− | ! colspan="3" style="text-align: center; font-weight:bold;" | Unstimulated
| |
− | ! colspan="3" style="text-align: center; font-weight:bold;" | A23187 (5 mM, 6h)
| |
− | ! colspan="3" style="text-align: center; font-weight:bold;" | UVR (15 mJ/cm2, 6h)
| |
− | |-
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center; font-weight:bold;" | Mean
| |
− | | style="text-align: center; font-weight:bold;" | SD
| |
− | | style="text-align: center; font-weight:bold;" | n
| |
− | | style="text-align: center; font-weight:bold;" | Mean
| |
− | | style="text-align: center; font-weight:bold;" | SD
| |
− | | style="text-align: center; font-weight:bold;" | n
| |
− | | style="text-align: center; font-weight:bold;" | Mean
| |
− | | style="text-align: center; font-weight:bold;" | SD
| |
− | | style="text-align: center; font-weight:bold;" | n
| |
− | |-
| |
− | | style="text-align: center;" | 15-LOX
| |
− | | style="text-align: center;" | 9.45
| |
− | | style="text-align: center;" | 0.065
| |
− | | style="text-align: center;" | 2
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | |-
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− | | style="text-align: center;" | 15-PGDH
| |
− | | style="text-align: center;" | 9.29
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− | | style="text-align: center;" | 0
| |
− | | style="text-align: center;" | 1
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" | 9.47
| |
− | | style="text-align: center;" | 0.27
| |
− | | style="text-align: center;" | 3
| |
− | |-
| |
− | | style="text-align: center;" | COX-1
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" | 11.04
| |
− | | style="text-align: center;" | 0.091
| |
− | | style="text-align: center;" | 2
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | |-
| |
− | | style="text-align: center;" | LTA4H
| |
− | | style="text-align: center;" | 12.17
| |
− | | style="text-align: center;" | 0.25
| |
− | | style="text-align: center;" | 3
| |
− | | style="text-align: center;" | 8.12
| |
− | | style="text-align: center;" | 0
| |
− | | style="text-align: center;" | 1
| |
− | | style="text-align: center;" | 12.14
| |
− | | style="text-align: center;" | 0.093
| |
− | | style="text-align: center;" | 3
| |
− | |-
| |
− | | style="text-align: center;" | PGES
| |
− | | style="text-align: center;" | 10.89
| |
− | | style="text-align: center;" | 0.23
| |
− | | style="text-align: center;" | 3
| |
− | | style="text-align: center;" | 13.6
| |
− | | style="text-align: center;" | 0.38
| |
− | | style="text-align: center;" | 2
| |
− | | style="text-align: center;" | 10.07
| |
− | | style="text-align: center;" | 0.12
| |
− | | style="text-align: center;" | 3
| |
− | |-
| |
− | | style="text-align: center;" | PHGPx
| |
− | | style="text-align: center;" | 7.49
| |
− | | style="text-align: center;" | 0
| |
− | | style="text-align: center;" | 1
| |
− | | style="text-align: center;" | 4.07
| |
− | | style="text-align: center;" | 5.76
| |
− | | style="text-align: center;" | 2
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | |-
| |
− | | style="text-align: center;" | TXAS
| |
− | | style="text-align: center;" | 8.74
| |
− | | style="text-align: center;" | 0.77
| |
− | | style="text-align: center;" | 2
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" |
| |
− | | style="text-align: center;" | 8.81
| |
− | | style="text-align: center;" | 0
| |
− | | style="text-align: center;" | 1
| |
− | |}
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