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| [[Welcome to the In-Silico Model of Cutaneous Lipids Wiki | Return to overview]] | | [[Welcome to the In-Silico Model of Cutaneous Lipids Wiki | Return to overview]] |
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| + | [[HaCaT keratinocytes|Return to HaCaT keratinocytes]] |
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| == Preliminary analysis of the proteomic dataset == | | == Preliminary analysis of the proteomic dataset == |
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| [[Image:Row_stats_of_H_all_-_use.jpg|none|thumb|500px|Proteomic analysis of AA cascade proteins in HaCaT keratinocytes. Results shown as the raw intensities from two independent experiments of A23187 stimulated (green triangle) and unstimulated HaCaT keratinocytes (red circle), and three independent experiments of UVR stimulated HaCaT keratinocytes (blue diamond).]] | | [[Image:Row_stats_of_H_all_-_use.jpg|none|thumb|500px|Proteomic analysis of AA cascade proteins in HaCaT keratinocytes. Results shown as the raw intensities from two independent experiments of A23187 stimulated (green triangle) and unstimulated HaCaT keratinocytes (red circle), and three independent experiments of UVR stimulated HaCaT keratinocytes (blue diamond).]] |
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− | |+ style="text-align: left;" |The raw intensity of proteins detected in HaCaT keratinocytes which were unstimulated and stimulated with A23187 and UVR. Data is presented as mean ± SD for dual injections of two independent experiments of A23187 stimulated and unstimulated HaCaT keratinocytes, and three independent experiments of UVR stimulated HaCaT keratinocytes.
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− | ! style="text-align: center;" |
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− | ! colspan="3" style="text-align: center; font-weight:bold;" | Unstimulated
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− | ! colspan="3" style="text-align: center; font-weight:bold;" | A23187 (5 mM, 6h)
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− | ! colspan="3" style="text-align: center; font-weight:bold;" | UVR (15 mJ/cm2, 6h)
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− | | style="text-align: center;" |
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− | | style="text-align: center; font-weight:bold;" | Mean
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− | | style="text-align: center; font-weight:bold;" | SD
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− | | style="text-align: center; font-weight:bold;" | n
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− | | style="text-align: center; font-weight:bold;" | Mean
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− | | style="text-align: center; font-weight:bold;" | SD
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− | | style="text-align: center; font-weight:bold;" | n
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− | | style="text-align: center; font-weight:bold;" | Mean
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− | | style="text-align: center; font-weight:bold;" | SD
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− | | style="text-align: center; font-weight:bold;" | n
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− | |-
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− | | style="text-align: center;" | 12(S)-LOX
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | |-
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− | | style="text-align: center;" | 15-LOX-1
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− | | style="text-align: center;" | 1.72E+03
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− | | style="text-align: center;" | 0.00E+00
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− | | style="text-align: center;" | 1
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" | 8.28E+02
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− | | style="text-align: center;" | 4.66E+02
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− | | style="text-align: center;" | 3
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− | |-
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− | | style="text-align: center;" | 5-LOX
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− | | style="text-align: center;" | 1.03E+03
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− | | style="text-align: center;" | 0.00E+00
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− | | style="text-align: center;" | 1
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− | | style="text-align: center;" | 5.39E+02
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− | | style="text-align: center;" | 0.00E+00
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− | | style="text-align: center;" | 1
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− | | style="text-align: center;" | 1.57E+03
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− | | style="text-align: center;" | 1.83E+03
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− | | style="text-align: center;" | 2
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− | |-
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− | | style="text-align: center;" | 5LOX/FLAP
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− | | style="text-align: center;" | 1.68E+03
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− | | style="text-align: center;" | 8.34E+02
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− | | style="text-align: center;" | 3
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− | | style="text-align: center;" | 2.16E+03
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− | | style="text-align: center;" | 2.13E+03
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− | | style="text-align: center;" | 2
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− | | style="text-align: center;" | 1.31E+03
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− | | style="text-align: center;" | 1.30E+03
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− | | style="text-align: center;" | 2
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− | | style="text-align: center;" | LTA4H
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− | | style="text-align: center;" | 6.33E+04
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− | | style="text-align: center;" | 1.05E+04
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− | | style="text-align: center;" | 4
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− | | style="text-align: center;" | 9.33E+03
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− | | style="text-align: center;" | 1.43E+04
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− | | style="text-align: center;" | 4
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− | | style="text-align: center;" | 7.25E+04
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− | | style="text-align: center;" | 9.57E+03
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− | | style="text-align: center;" | 4
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− | | style="text-align: center;" | PHGPx
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− | | style="text-align: center;" | 3.42E+03
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− | | style="text-align: center;" | 0.00E+00
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− | | style="text-align: center;" | 1
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− | | style="text-align: center;" | 2.35E+03
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− | | style="text-align: center;" | 2.42E+03
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− | | style="text-align: center;" | 3
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | |-
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− | | style="text-align: center;" | COX-1
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− | | style="text-align: center;" | 3.05E+04
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− | | style="text-align: center;" | 0.00E+00
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− | | style="text-align: center;" | 1
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− | | style="text-align: center;" | 1.52E+04
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− | | style="text-align: center;" | 1.32E+04
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− | | style="text-align: center;" | 4
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− | | style="text-align: center;" | 3.43E+03
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− | | style="text-align: center;" | 4.61E+03
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− | | style="text-align: center;" | 6
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− | | style="text-align: center;" | PGFS (AKR13)
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− | | style="text-align: center;" | 1.24E+03
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− | | style="text-align: center;" | 0.00E+00
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− | | style="text-align: center;" | 1
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− | | style="text-align: center;" | 1.46E+03
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− | | style="text-align: center;" | 0.00E+00
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− | | style="text-align: center;" | 1
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | |-
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− | | style="text-align: center;" | H-PGDS
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" | 3.61E+02
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− | | style="text-align: center;" | 0.00E+00
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− | | style="text-align: center;" | 1
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− | | style="text-align: center;" | 2.71E+03
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− | | style="text-align: center;" | 0.00E+00
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− | | style="text-align: center;" | 1
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− | | style="text-align: center;" | cPGES
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− | | style="text-align: center;" | 1.97E+05
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− | | style="text-align: center;" | 1.05E+05
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− | | style="text-align: center;" | 4
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− | | style="text-align: center;" | 7.12E+04
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− | | style="text-align: center;" | 1.72E+04
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− | | style="text-align: center;" | 4
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− | | style="text-align: center;" | 2.48E+05
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− | | style="text-align: center;" | 9.60E+04
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− | | style="text-align: center;" | 6
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− | | style="text-align: center;" | mPGES-2
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− | | style="text-align: center;" | 1.49E+04
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− | | style="text-align: center;" | 5.91E+03
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− | | style="text-align: center;" | 4
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− | | style="text-align: center;" | 5.48E+04
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− | | style="text-align: center;" | 1.81E+04
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− | | style="text-align: center;" | 4
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− | | style="text-align: center;" | 1.57E+04
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− | | style="text-align: center;" | 2.77E+03
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− | | style="text-align: center;" | 6
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− | | style="text-align: center;" | 15-PGDH
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− | | style="text-align: center;" | 2.75E+03
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− | | style="text-align: center;" | 2.22E+02
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− | | style="text-align: center;" | 2
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− | | style="text-align: center;" | 1.83E+03
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− | | style="text-align: center;" | 1.37E+03
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− | | style="text-align: center;" | 3
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− | | style="text-align: center;" | 2.93E+03
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− | | style="text-align: center;" | 1.26E+03
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− | | style="text-align: center;" | 6
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− | | style="text-align: center;" | PTGR-2
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− | | style="text-align: center;" | 9.37E+03
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− | | style="text-align: center;" | 6.40E+03
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− | | style="text-align: center;" | 4
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− | | style="text-align: center;" | 6.17E+03
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− | | style="text-align: center;" | 4.56E+03
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− | | style="text-align: center;" | 3
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− | | style="text-align: center;" | 2.24E+03
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− | | style="text-align: center;" | 1.53E+03
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− | | style="text-align: center;" | 5
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− | | style="text-align: center;" | TXAS
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− | | style="text-align: center;" | 8.06E+03
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− | | style="text-align: center;" | 5.75E+03
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− | | style="text-align: center;" | 4
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− | | style="text-align: center;" | 1.31E+03
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− | | style="text-align: center;" | 5.89E+02
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− | | style="text-align: center;" | 2
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− | | style="text-align: center;" | 2.88E+03
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− | | style="text-align: center;" | 1.87E+03
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− | | style="text-align: center;" | 4
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− | |}
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| == Further analysis of the proteomic dataset == | | == Further analysis of the proteomic dataset == |
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| [[Image:Proteomic_analysis_-_dave.jpg|none|thumb|500px|In-depth proteomic analysis of AA cascade proteins in HaCaT keratinocytes. Results shown are from two independent experiments of A23187 stimulated (green triangle) and unstimulated HaCaT keratinocytes (red circle), and three independent experiments of UVR stimulated HaCaT keratinocytes (blue diamond).]] | | [[Image:Proteomic_analysis_-_dave.jpg|none|thumb|500px|In-depth proteomic analysis of AA cascade proteins in HaCaT keratinocytes. Results shown are from two independent experiments of A23187 stimulated (green triangle) and unstimulated HaCaT keratinocytes (red circle), and three independent experiments of UVR stimulated HaCaT keratinocytes (blue diamond).]] |
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− | {| class="wikitable"
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− | |+ style="text-align: left;" |The Log2 intensity of proteins detected in HaCaT keratinocytes which were unstimulated and stimulated with A23187 and UVR. Data is presented as mean ± SD for two independent experiments of A23187 stimulated and unstimulated HaCaT keratinocytes, and three independent experiments of UVR stimulated HaCaT keratinocytes.
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− | ! style="text-align: center;" |
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− | ! colspan="3" style="text-align: center; font-weight:bold;" | Unstimulated
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− | ! colspan="3" style="text-align: center; font-weight:bold;" | A23187 (5 mM, 6h)
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− | ! colspan="3" style="text-align: center; font-weight:bold;" | UVR (15 mJ/cm2, 6h)
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− | | style="text-align: center;" |
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− | | style="text-align: center; font-weight:bold;" | Mean
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− | | style="text-align: center; font-weight:bold;" | SD
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− | | style="text-align: center; font-weight:bold;" | n
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− | | style="text-align: center; font-weight:bold;" | Mean
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− | | style="text-align: center; font-weight:bold;" | SD
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− | | style="text-align: center; font-weight:bold;" | n
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− | | style="text-align: center; font-weight:bold;" | Mean
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− | | style="text-align: center; font-weight:bold;" | SD
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− | | style="text-align: center; font-weight:bold;" | n
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− | | style="text-align: center;" | 15-LOX
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− | | style="text-align: center;" | 9.45
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− | | style="text-align: center;" | 0.065
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− | | style="text-align: center;" | 2
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | |-
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− | | style="text-align: center;" | 15-PGDH
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− | | style="text-align: center;" | 9.29
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− | | style="text-align: center;" | 0
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− | | style="text-align: center;" | 1
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" | 9.47
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− | | style="text-align: center;" | 0.27
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− | | style="text-align: center;" | 3
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− | |-
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− | | style="text-align: center;" | COX-1
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" | 11.04
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− | | style="text-align: center;" | 0.091
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− | | style="text-align: center;" | 2
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | |-
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− | | style="text-align: center;" | LTA4H
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− | | style="text-align: center;" | 12.17
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− | | style="text-align: center;" | 0.25
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− | | style="text-align: center;" | 3
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− | | style="text-align: center;" | 8.12
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− | | style="text-align: center;" | 0
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− | | style="text-align: center;" | 1
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− | | style="text-align: center;" | 12.14
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− | | style="text-align: center;" | 0.093
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− | | style="text-align: center;" | 3
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− | | style="text-align: center;" | PGES
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− | | style="text-align: center;" | 10.89
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− | | style="text-align: center;" | 0.23
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− | | style="text-align: center;" | 3
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− | | style="text-align: center;" | 13.6
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− | | style="text-align: center;" | 0.38
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− | | style="text-align: center;" | 2
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− | | style="text-align: center;" | 10.07
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− | | style="text-align: center;" | 0.12
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− | | style="text-align: center;" | 3
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− | |-
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− | | style="text-align: center;" | PHGPx
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− | | style="text-align: center;" | 7.49
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− | | style="text-align: center;" | 0
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− | | style="text-align: center;" | 1
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− | | style="text-align: center;" | 4.07
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− | | style="text-align: center;" | 5.76
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− | | style="text-align: center;" | 2
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | |-
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− | | style="text-align: center;" | TXAS
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− | | style="text-align: center;" | 8.74
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− | | style="text-align: center;" | 0.77
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− | | style="text-align: center;" | 2
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" |
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− | | style="text-align: center;" | 8.81
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− | | style="text-align: center;" | 0
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− | | style="text-align: center;" | 1
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− | |}
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