HaCaT MTT Assay Results
Revision as of 13:58, 31 May 2019 by Muttley93 (talk | contribs) (Created page with " Return to overview In this study, the aim was to identify optimal conditions for the following experiments. Thes...")
In this study, the aim was to identify optimal conditions for the following experiments. These assays were performed using the MTT assay.
Optimum seed density
HaCaT keratinocytes were seeded at set densities between 0.4-44 x104 cells/cm2 in order to create a growth curve (Figure *). The exponential growth phase was identified between 0.4 x10\sup4 and 4 x10^4 cells/cm2. As a result, a value in the centre of the exponential growth phase was selected (2 x10^4 cells/cm2) and used in all future experiments.
