Difference between revisions of "HaCaT Fatty acid analysis Results"
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[[HaCaT keratinocytes|Return to HaCaT keratinocytes]] | [[HaCaT keratinocytes|Return to HaCaT keratinocytes]] | ||
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+ | == Summary Table == | ||
+ | |||
+ | {| class="wikitable" | ||
+ | |+ style="text-align: left;" |The estimated AA levels in HaCaT keratinocytes (unstimulated, A23187 (5µM), COX inhibited and A23187 (5 µM), ATP (2 mM) and UVR (15 mJ/cm2)) for 0.5, 1, 3 and 6h. Analysis was performed using GC-FID. Results are expressed mean ± SD (ug/106 cells) of three independent experiments. | ||
+ | |- | ||
+ | ! rowspan="2" style="text-align: center; font-weight:bold;" | Time (h) | ||
+ | ! colspan="5" style="text-align: center; font-weight:bold;" | Experiment | ||
+ | |- | ||
+ | | style="text-align: center; font-weight:bold;" | Unstimulated | ||
+ | | style="text-align: center; font-weight:bold;" | A23187 | ||
+ | | style="text-align: center; font-weight:bold;" | Indomethacin + A23187 | ||
+ | | style="text-align: center; font-weight:bold;" | UVR | ||
+ | | style="text-align: center; font-weight:bold;" | ATP | ||
+ | |- | ||
+ | | style="text-align: center;" | 0 | ||
+ | | style="text-align: center;" | 2.87 ± 0.28 | ||
+ | | style="text-align: center;" | 2.87 ± 0.28 | ||
+ | | style="text-align: center;" | 2.87 ± 0.28 | ||
+ | | style="text-align: center;" | 2.87 ± 0.28 | ||
+ | | style="text-align: center;" | 2.87 ± 0.28 | ||
+ | |- | ||
+ | | style="text-align: center;" | 0.5 | ||
+ | | style="text-align: center;" | 2.68 ± 0.49 | ||
+ | | style="text-align: center;" | 0.28 ± 0.07 | ||
+ | | style="text-align: center;" | 4.18 ± 0.47 | ||
+ | | style="text-align: center;" | 2.41 ± 0.70 | ||
+ | | style="text-align: center;" | 1.27 ± 0.23 | ||
+ | |- | ||
+ | | style="text-align: center;" | 1 | ||
+ | | style="text-align: center;" | 2.57 ± 1.25 | ||
+ | | style="text-align: center;" | 0.21 ± 0.09 | ||
+ | | style="text-align: center;" | 3.42 ± 0.47 | ||
+ | | style="text-align: center;" | 2.15 ± 0.72 | ||
+ | | style="text-align: center;" | 1.44 ± 0.41 | ||
+ | |- | ||
+ | | style="text-align: center;" | 3 | ||
+ | | style="text-align: center;" | 2.51 ± 0.82 | ||
+ | | style="text-align: center;" | 0.25 ± 0.20 | ||
+ | | style="text-align: center;" | 1.98 ± 0.34 | ||
+ | | style="text-align: center;" | 1.74 ± 0.77 | ||
+ | | style="text-align: center;" | 1.15 ± 0.20 | ||
+ | |- | ||
+ | | style="text-align: center;" | 6 | ||
+ | | style="text-align: center;" | 2.91 ± 0.22 | ||
+ | | style="text-align: center;" | 0.28 ± 0.35 | ||
+ | | style="text-align: center;" | 1.13 ± 0.49 | ||
+ | | style="text-align: center;" | 0.79 ± 0.82 | ||
+ | | style="text-align: center;" | 1.47 ± 0.28 | ||
+ | |} | ||
== Estimation of cellular arachidonic acid in unstimulated cells == | == Estimation of cellular arachidonic acid in unstimulated cells == |
Revision as of 15:15, 31 May 2019
Contents
- 1 Summary Table
- 2 Estimation of cellular arachidonic acid in unstimulated cells
- 3 Estimation of cellular arachidonic acid following stimulation with calcium ionophore (A23187)
- 4 Estimation of cellular arachidonic acid following stimulation with ATP
- 5 Estimation of cellular arachidonic acid following stimulation with UVR
Summary Table
Time (h) | Experiment | ||||
---|---|---|---|---|---|
Unstimulated | A23187 | Indomethacin + A23187 | UVR | ATP | |
0 | 2.87 ± 0.28 | 2.87 ± 0.28 | 2.87 ± 0.28 | 2.87 ± 0.28 | 2.87 ± 0.28 |
0.5 | 2.68 ± 0.49 | 0.28 ± 0.07 | 4.18 ± 0.47 | 2.41 ± 0.70 | 1.27 ± 0.23 |
1 | 2.57 ± 1.25 | 0.21 ± 0.09 | 3.42 ± 0.47 | 2.15 ± 0.72 | 1.44 ± 0.41 |
3 | 2.51 ± 0.82 | 0.25 ± 0.20 | 1.98 ± 0.34 | 1.74 ± 0.77 | 1.15 ± 0.20 |
6 | 2.91 ± 0.22 | 0.28 ± 0.35 | 1.13 ± 0.49 | 0.79 ± 0.82 | 1.47 ± 0.28 |
Estimation of cellular arachidonic acid in unstimulated cells
Estimation of cellular arachidonic acid following stimulation with calcium ionophore (A23187)
Estimation of cellular arachidonic acid following stimulation with ATP