Difference between revisions of "HaCaT Fatty acid analysis Results"

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[[HaCaT keratinocytes|Return to HaCaT keratinocytes]]
 
[[HaCaT keratinocytes|Return to HaCaT keratinocytes]]
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== Summary Table ==
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{| class="wikitable"
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|+  style="text-align: left;" |The estimated AA levels in HaCaT keratinocytes (unstimulated, A23187 (5µM), COX inhibited and A23187 (5 µM), ATP (2 mM) and UVR (15 mJ/cm2)) for 0.5, 1, 3 and 6h. Analysis was performed using GC-FID. Results are expressed mean ± SD (ug/106 cells) of three independent experiments.
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|-
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! rowspan="2" style="text-align: center; font-weight:bold;" | Time (h)
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! colspan="5" style="text-align: center; font-weight:bold;" | Experiment
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|-
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| style="text-align: center; font-weight:bold;" | Unstimulated
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| style="text-align: center; font-weight:bold;" | A23187
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| style="text-align: center; font-weight:bold;" | Indomethacin + A23187
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| style="text-align: center; font-weight:bold;" | UVR
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| style="text-align: center; font-weight:bold;" | ATP
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|-
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| style="text-align: center;" | 0
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| style="text-align: center;" | 2.87 ± 0.28
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| style="text-align: center;" | 2.87 ± 0.28
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| style="text-align: center;" | 2.87 ± 0.28
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| style="text-align: center;" | 2.87 ± 0.28
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| style="text-align: center;" | 2.87 ± 0.28
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|-
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| style="text-align: center;" | 0.5
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| style="text-align: center;" | 2.68 ± 0.49
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| style="text-align: center;" | 0.28 ± 0.07
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| style="text-align: center;" | 4.18 ± 0.47
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| style="text-align: center;" | 2.41 ± 0.70
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| style="text-align: center;" | 1.27 ± 0.23
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|-
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| style="text-align: center;" | 1
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| style="text-align: center;" | 2.57 ± 1.25
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| style="text-align: center;" | 0.21 ± 0.09
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| style="text-align: center;" | 3.42 ± 0.47
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| style="text-align: center;" | 2.15 ± 0.72
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| style="text-align: center;" | 1.44 ± 0.41
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|-
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| style="text-align: center;" | 3
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| style="text-align: center;" | 2.51 ± 0.82
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| style="text-align: center;" | 0.25 ± 0.20
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| style="text-align: center;" | 1.98 ± 0.34
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| style="text-align: center;" | 1.74 ± 0.77
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| style="text-align: center;" | 1.15 ± 0.20
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|-
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| style="text-align: center;" | 6
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| style="text-align: center;" | 2.91 ± 0.22
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| style="text-align: center;" | 0.28 ± 0.35
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| style="text-align: center;" | 1.13 ± 0.49
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| style="text-align: center;" | 0.79 ± 0.82
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| style="text-align: center;" | 1.47 ± 0.28
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|}
  
 
== Estimation of cellular arachidonic acid in unstimulated cells ==  
 
== Estimation of cellular arachidonic acid in unstimulated cells ==  

Revision as of 15:15, 31 May 2019

Return to HaCaT keratinocytes

Summary Table

The estimated AA levels in HaCaT keratinocytes (unstimulated, A23187 (5µM), COX inhibited and A23187 (5 µM), ATP (2 mM) and UVR (15 mJ/cm2)) for 0.5, 1, 3 and 6h. Analysis was performed using GC-FID. Results are expressed mean ± SD (ug/106 cells) of three independent experiments.
Time (h) Experiment
Unstimulated A23187 Indomethacin + A23187 UVR ATP
0 2.87 ± 0.28 2.87 ± 0.28 2.87 ± 0.28 2.87 ± 0.28 2.87 ± 0.28
0.5 2.68 ± 0.49 0.28 ± 0.07 4.18 ± 0.47 2.41 ± 0.70 1.27 ± 0.23
1 2.57 ± 1.25 0.21 ± 0.09 3.42 ± 0.47 2.15 ± 0.72 1.44 ± 0.41
3 2.51 ± 0.82 0.25 ± 0.20 1.98 ± 0.34 1.74 ± 0.77 1.15 ± 0.20
6 2.91 ± 0.22 0.28 ± 0.35 1.13 ± 0.49 0.79 ± 0.82 1.47 ± 0.28

Estimation of cellular arachidonic acid in unstimulated cells

Estimation of AA in unstimulated HaCaT keratinocytes over 6h. The data presented is from three independent experiments.

Estimation of cellular arachidonic acid following stimulation with calcium ionophore (A23187)

Estimation of AA in HaCaT keratinocytes stimulated with A23187 (5 µM) for 6h. The data presented is from three independent experiments.

Estimation of cellular arachidonic acid following stimulation with ATP

Estimation of AA in HaCaT keratinocytes stimulated with ATP (2 mM) for 6h. The data presented is from three independent experiments.


Estimation of cellular arachidonic acid following stimulation with UVR

Estimation of AA in HaCaT keratinocytes over 6h following stimulation with UVR (15 mJ/cm2). The data presented is from three independent experiments.
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