Difference between revisions of "HaCaT Eicosanoid Analysis Results"

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[[Image:K03_summary.jpg|none|thumb|500px|Lipid mediator levels in unstimulated (red) and stimulated with A23187 (5 µM) (blue) HaCaT keratinocytes over 6h. Where (A) PGE2, (B) 15-keto-PGE2, (C) 13,14-dihydro-15-keto-PGE2, (D) PGF2α, (E) 12-HETE and (D) 15-HETE. The data presented is from three independent experiments, each with a technical replicate.  ]]

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Calcium Ionophore

AA-derived lipid mediators detected in the supernatant of HaCaT keratinocytes treated with A23187 (5 µM), for 0.5, 1, 3 and 6h. Analysis was performed using UPLC-ESI-MS/MS. Results are expressed mean ± SD (pg/106 cells) of three independent experiments.
Time (h) Mediator
PGE2 15-keto-PGE2 13,14-dihydro-15-keto-PGE2 PGF2α 12-HETE 15-HETE
0 9.45 ± 7.56 0.50 ± 0.00 2.00 ± 0.00 7.00 ± 0.00 6.44 ± 4.40 3.72 ± 1.08
0.5 601.26 ± 162.02 65.52 ± 57.95 211.38 ± 61.05 106.06 ± 39.89 19.49 ± 10.36 163.26 ± 143.10
1 14371.34 ± 858.76 2136.87 ± 588.92 2461.84 ± 452.79 2787.86 ± 489.04 363.25 ± 96.91 4050.14 ± 2998.16
3 79576.88 ± 15355.19 12230.83 ± 2179.66 11025.93 ± 2287.76 19276.46 ± 3732.43 4872.78 ± 849.95 41799.69 ± 31552.30
6 111115.14 ± 32976.86 18619.32 ± 5691.99 15799.31 ± 5867.25 34965.99 ± 11250.10 7156.92 ± 1550.86 55738.98 ± 40130.05


Lipid mediator levels in unstimulated (red) and stimulated with A23187 (5 µM) (blue) HaCaT keratinocytes over 6h. Where (A) PGE2, (B) 15-keto-PGE2, (C) 13,14-dihydro-15-keto-PGE2, (D) PGF2α, (E) 12-HETE and (D) 15-HETE. The data presented is from three independent experiments, each with a technical replicate.
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