Difference between revisions of "HaCaT Eicosanoid Analysis Results"
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| − | == | + | == Calcium Ionophore == |
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| + | {| class="wikitable" | ||
| + | |+ style="text-align: left;" |AA-derived lipid mediators detected in the supernatant of HaCaT keratinocytes treated with A23187 (5 µM), for 0.5, 1, 3 and 6h. Analysis was performed using UPLC-ESI-MS/MS. Results are expressed mean ± SD (pg/106 cells) of three independent experiments. | ||
| + | |- | ||
| + | ! rowspan="2" style="text-align: center; font-weight:bold;" | Time (h) | ||
| + | ! colspan="6" style="text-align: center; font-weight:bold;" | Mediator | ||
| + | |- | ||
| + | | style="text-align: center; font-weight:bold;" | PGE2 | ||
| + | | style="text-align: center; font-weight:bold;" | 15-keto-PGE2 | ||
| + | | style="text-align: center; font-weight:bold;" | 13,14-dihydro-15-keto-PGE2 | ||
| + | | style="text-align: center; font-weight:bold;" | PGF2α | ||
| + | | style="text-align: center; font-weight:bold;" | 12-HETE | ||
| + | | 15-HETE | ||
| + | |- | ||
| + | | style="text-align: center;" | 0 | ||
| + | | style="text-align: center;" | 9.45 ± 7.56 | ||
| + | | style="text-align: center;" | 0.50 ± 0.00 | ||
| + | | style="text-align: center;" | 2.00 ± 0.00 | ||
| + | | style="text-align: center;" | 7.00 ± 0.00 | ||
| + | | style="text-align: center;" | 6.44 ± 4.40 | ||
| + | | 3.72 ± 1.08 | ||
| + | |- | ||
| + | | style="text-align: center;" | 0.5 | ||
| + | | style="text-align: center;" | 601.26 ± 162.02 | ||
| + | | style="text-align: center;" | 65.52 ± 57.95 | ||
| + | | style="text-align: center;" | 211.38 ± 61.05 | ||
| + | | style="text-align: center;" | 106.06 ± 39.89 | ||
| + | | style="text-align: center;" | 19.49 ± 10.36 | ||
| + | | 163.26 ± 143.10 | ||
| + | |- | ||
| + | | style="text-align: center;" | 1 | ||
| + | | style="text-align: center;" | 14371.34 ± 858.76 | ||
| + | | style="text-align: center;" | 2136.87 ± 588.92 | ||
| + | | style="text-align: center;" | 2461.84 ± 452.79 | ||
| + | | style="text-align: center;" | 2787.86 ± 489.04 | ||
| + | | style="text-align: center;" | 363.25 ± 96.91 | ||
| + | | 4050.14 ± 2998.16 | ||
| + | |- | ||
| + | | style="text-align: center;" | 3 | ||
| + | | style="text-align: center;" | 79576.88 ± 15355.19 | ||
| + | | style="text-align: center;" | 12230.83 ± 2179.66 | ||
| + | | style="text-align: center;" | 11025.93 ± 2287.76 | ||
| + | | style="text-align: center;" | 19276.46 ± 3732.43 | ||
| + | | style="text-align: center;" | 4872.78 ± 849.95 | ||
| + | | 41799.69 ± 31552.30 | ||
| + | |- | ||
| + | | style="text-align: center;" | 6 | ||
| + | | style="text-align: center;" | 111115.14 ± 32976.86 | ||
| + | | style="text-align: center;" | 18619.32 ± 5691.99 | ||
| + | | style="text-align: center;" | 15799.31 ± 5867.25 | ||
| + | | style="text-align: center;" | 34965.99 ± 11250.10 | ||
| + | | style="text-align: center;" | 7156.92 ± 1550.86 | ||
| + | | 55738.98 ± 40130.05 | ||
| + | |} | ||
Revision as of 15:19, 31 May 2019
Calcium Ionophore
| Time (h) | Mediator | |||||
|---|---|---|---|---|---|---|
| PGE2 | 15-keto-PGE2 | 13,14-dihydro-15-keto-PGE2 | PGF2α | 12-HETE | 15-HETE | |
| 0 | 9.45 ± 7.56 | 0.50 ± 0.00 | 2.00 ± 0.00 | 7.00 ± 0.00 | 6.44 ± 4.40 | 3.72 ± 1.08 |
| 0.5 | 601.26 ± 162.02 | 65.52 ± 57.95 | 211.38 ± 61.05 | 106.06 ± 39.89 | 19.49 ± 10.36 | 163.26 ± 143.10 |
| 1 | 14371.34 ± 858.76 | 2136.87 ± 588.92 | 2461.84 ± 452.79 | 2787.86 ± 489.04 | 363.25 ± 96.91 | 4050.14 ± 2998.16 |
| 3 | 79576.88 ± 15355.19 | 12230.83 ± 2179.66 | 11025.93 ± 2287.76 | 19276.46 ± 3732.43 | 4872.78 ± 849.95 | 41799.69 ± 31552.30 |
| 6 | 111115.14 ± 32976.86 | 18619.32 ± 5691.99 | 15799.31 ± 5867.25 | 34965.99 ± 11250.10 | 7156.92 ± 1550.86 | 55738.98 ± 40130.05 |
