Difference between revisions of "Fructose 1,6 bisphosphate aldolase"
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==Parameters with uncertainty== | ==Parameters with uncertainty== | ||
− | *The value for <math>V_{mf}</math> is collected from Hernandez ''et. al.'' <ref name="Hernandez_2006"></ref>. The <math>V_{mr}</math> is calcualted from the sampled <math>V_{mf}</math>, <math>Km_{Gly3P}</math>, <math>Km_{DHAP}</math> and <math>K_{eq}</math> values using the Haldane equation. '''Alternatively''' the reported value from AS-30D can be considered. The standard deviation can be calculated based on the same ratio of <math>V_{mf}</math>. However, as it is from a different species an extra 20% of uncertainty will be added. This gives the value <math>V_{mf}=0.063 \pm 0.028 </math><math>U\cdot(\text{mg protein})^{-1}</math>. Converting these value to <math>mM\cdot(min)^{-1}</math> gives 0.013 <math>mM\cdot(min)^{-1}</math>. But in the model they used the value of 0.08 <math>mM\cdot(min)^{-1}</math>. Considering these values the value of <math>V_{mf}</math> is | + | *The value for <math>V_{mf}</math> is collected from Hernandez ''et. al.'' <ref name="Hernandez_2006"></ref>. The <math>V_{mr}</math> is calcualted from the sampled <math>V_{mf}</math>, <math>Km_{Gly3P}</math>, <math>Km_{DHAP}</math> and <math>K_{eq}</math> values using the Haldane equation. '''Alternatively''' the reported value from AS-30D can be considered. The standard deviation can be calculated based on the same ratio of <math>V_{mf}</math>. However, as it is from a different species an extra 20% of uncertainty will be added. This gives the value <math>V_{mf}=0.063 \pm 0.028 </math><math>U\cdot(\text{mg protein})^{-1}</math>. Converting these value to <math>mM\cdot(min)^{-1}</math> gives 0.013 <math>mM\cdot(min)^{-1}</math>. But in the model they used the value of 0.08 <math>mM\cdot(min)^{-1}</math>. Considering these values the value of <math>V_{mf}</math> is <math>0.0465 \pm 0.047 </math> <math>mM\cdot(min)^{-1}</math> |
*The value for <math>Km_{Fru1,6BP}, Km_{Gly3P}, Km_{DHAP}</math> are collected from Ali D. Malay ''et. al.'' <ref name="Ali">Malay AD, Procious SL, Tolan DR. (2002). ''The temperature dependence of activity and structure for most prevalent mutant aldolase B associated with hereditary fructose intolerance'', Arch BiochemBiophys 408: 295–304.</ref> for wildtype Aldolase B gene at <math>30^{\circ}</math>C. | *The value for <math>Km_{Fru1,6BP}, Km_{Gly3P}, Km_{DHAP}</math> are collected from Ali D. Malay ''et. al.'' <ref name="Ali">Malay AD, Procious SL, Tolan DR. (2002). ''The temperature dependence of activity and structure for most prevalent mutant aldolase B associated with hereditary fructose intolerance'', Arch BiochemBiophys 408: 295–304.</ref> for wildtype Aldolase B gene at <math>30^{\circ}</math>C. | ||
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|<math>V_{mf}</math> | |<math>V_{mf}</math> | ||
− | |<math>0.0465 \pm 0.047 | + | |<math>0.0465 \pm 0.047 </math> <ref name="Hernandez_2006"> Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, ''et al.'' (2006). ''Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase''. FEBS J., 273 , pp. 1975–1988([http://dx.doi.org/doi:10.1111/j.1742-4658.2006.05214.x doi]) </ref> |
− | |<math> | + | |<math>mM\cdot(min)^{-1}</math> |
|Hela cell line | |Hela cell line | ||
|rowspan="5"| | |rowspan="5"| |
Revision as of 13:06, 22 September 2014
This enzyme splits fructose 1, 6-bisphosphate into two sugars that are isomers of each other. These two sugars are Dihydroxyacetone phosphate (DHAP) and Glyceraldehyde 3-phosphate (Gly3P).
Contents
Chemical equation
Rate equation
Reversible Uni-Bi Michaelis-Menten is used. [1]
Modified rate law to take into consideration the equilibrium constant
Parameters
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
0.08 [2] | Hela cell line | |||
0.063[2] | Rodent AS-30D hepatoma | |||
0.009[2] | mM | Hela cell line | ||
0.16[2] | mM | Rodent AS-30D hepatoma | ||
0.08[2] | mM | Rodent AS-30D hepatoma |
Parameters with uncertainty
- The value for is collected from Hernandez et. al. [2]. The is calcualted from the sampled , , and values using the Haldane equation. Alternatively the reported value from AS-30D can be considered. The standard deviation can be calculated based on the same ratio of . However, as it is from a different species an extra 20% of uncertainty will be added. This gives the value . Converting these value to gives 0.013 . But in the model they used the value of 0.08 . Considering these values the value of is Failed to parse (Cannot store math image on filesystem.): 0.0465 \pm 0.047
- The value for are collected from Ali D. Malay et. al. [3] for wildtype Aldolase B gene at C.
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
Failed to parse (Cannot store math image on filesystem.): 0.0465 \pm 0.047 [2] | Hela cell line | |||
Sampled based on Haldane equation. Alternative: |
||||
mM | Human cell | |||
mM | Human cell | |||
mM | Human cell |
Equilibrium constant
Equilibrium constant | Conditions | Source |
---|---|---|
0.10 | pH=7, T=25°C | Voet et al.[4] from Newshole et al. (1973) [5]p 97: , Failed to parse (Cannot store math image on filesystem.): Keq = exp(-\frac{\Delta G'}{RT}) = exp(\frac{-22800}{8.31*298.15}) \approx 0.00010 |
0.067 | pH=7, T=25°C | Lehninger, (1975)[6] p 407: , Failed to parse (Cannot store math image on filesystem.): Keq = exp(-\frac{\Delta G'}{RT}) = exp(\frac{-23800}{8.31*298.15}) \approx 6.7326E-005 |
- Taking average of these two values give Failed to parse (Cannot store math image on filesystem.): 8.40E-005 \pm 0.000023
References
- ↑ Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
- ↑ Malay AD, Procious SL, Tolan DR. (2002). The temperature dependence of activity and structure for most prevalent mutant aldolase B associated with hereditary fructose intolerance, Arch BiochemBiophys 408: 295–304.
- ↑ Voet, D., Voet., J.G. and Pratt, C. W. (1999) Fundamentals of biochemistry, Wiley
- ↑ Newshole, E.A. and Stuart, C. (1973) Regulation in Metabolism, Wiley
- ↑ Lehninger, A.L. (1975) Biochemistry (2nd edn), Worth