Dephosphorylation

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Binding Reaction

$\text{phosphorylated-enzyme} \rightleftharpoons \text{unphosphorylated-enzyme}$

Kinetic equation

$\frac{v_{max} \cdot [\text{phosphorylated-enzyme}] \cdot (1-\frac{[\text{unphosphorylated-enzyme}]}{[\text{phosphorylated-enzyme}] \cdot K_{eq}})}{1 + \frac{[\text{phosphorylated-enzyme}]}{Km_{\text{phosphorylated-enzyme}}} \cdot \frac{[\text{unphosphorylated-enzyme}]}{Km_{\text{unphosphorylated-enzyme}}}}$

final Parameter

$v_{max} = 1.13 \cdot 10^{2} \pm 3.09 \cdot 10^{2}\ \frac{1}{s}$

$K_{m} = 3.83 \cdot 10^{-3} \pm 1.40 \cdot 10^{-2}\ \mu M$

Parameter

Notes	Data
There are three main deophosphatases, phosphoserine phosphatase (EC 3.1.3.3), phosphoprotein phosphatase (EC 3.1.3.16), protein-tyrosine phosphatase (3.1.3.48). The data available in BRENDA is used to calculate the average and standard deviation.	EC 3.1.3.3 - phosphoserine phosphatase EC 3.1.3.16 - phosphoprotein phosphatase EC 3.1.3.48 - protein-tyrosine phosphatase Km [mM] average all 0.75 7.43 3.31 std 1.29 27.74 12.90 turnover number [1/s] average all 1.62*102 1.09 * 102 67.8 std 359.51 210.26 256.88