Degradation of R

The ScbR monomer (R) degrades.

Chemical equation

$R\rightarrow \varnothing$

Rate equation

$r= d_{R}\cdot[R]$

Parameters

The parameter of this reaction is the degradation rate of R ($d_{R}$).

Name	Value	Units	Value in previous GBL models	Remarks-Reference
$d_{R}$	$0.03-0.4$	$min^{-1}$	$0.004 s^{-1}$ $0.36 s^{-1}$ (Range tested: $10^{-7}-10^{-1} s^{-1}$) (Bistability range: $0.0022-0.0067 s^{-1}$ and $0.09-0.38 s^{-1}$)	In a study on global analysis of mRNA decay in gram positive bacteria by RNA-Seq, Kristoffersen et al. reported a range of values for the half-life ($t_{1/2}$) of mRNA in B. cereus between $1.9-5.6 min$, with a median of $2.4 min$. From these values we calculated the mRNA degradation rate of scbR as per $d_{mR}= \frac{ln(2)}{t_{1/2}}$, which resulted in a degradation rate constant value range $0.124-0.365 min^{-1}$ and a median $0.29 min^{-1}$. Kristoffersen et al. 2012

Name	Value	Units	Origin	Remarks
$d_{R}$	$0.000833-0.001667$	$min^{-1}$	Degradation rates of proteins in S. coelicolor based on radioactive decay

Parameters with uncertainty

The most plausible parameter value for the $d_{R}$ is decided to be $0.00118 min^{-1}$ and the confidence interval $1.41$. This means that the mode of the PDF is 0.00118 and the range where 95% of the values are found is between $0.8369\cdot 10^{-3}$ and $1.67 \cdot 10^{-3}min^{-1}$.

The probability distribution for the parameter, adjusted accordingly in order to reflect the above values, is the following:

The location and scale parameters of the distribution are:

Parameter	μ	σ
$d_{R}$	$-6.7120$	$0.174$