CMK
Revision as of 22:20, 7 March 2017 by Aliah.hawari (talk | contribs)
Contents
The reaction
4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinase (CMK) catalyses the production of CDP-ME2P from CDP-ME. This ATP-dependent reaction is the fourth step in the non-mevalonate or methylerythritol-phosphate (MEP) pathway.
Equation Rate
CMK is modelled reversible using random bi-bi generic Michaelis-Menten equation [1]
where :
Parameter | Description | Units |
---|---|---|
VCMK | Reaction rate for CMK | μM/min |
Kcatforward | Forward turnover number | min-1 |
KmCDPME | Michaelis-Menten constant for CDPME | μM |
KmATP | Michaelis-Menten constant for ATP | μM |
KmCDPME2P | Michaelis-Menten constant for CDPME2P | μM |
KmADP | Michaelis-Menten constant for ADP | μM |
Keq | Equilibrium constant | unitless |
[CDPME] | CDPME concentration | μM |
[ATP] | ATP concentration | μM |
[CDPME2P] | CDPME2P concentration | μM |
[ADP] | ADP concentration | μM |
Parameters
To model CMK using the equation above, a total of 6 parameters are required. For each of this parameter, a lognormal distribution is generated using our PROMENADE pipeline. To include all uncertainties surrounding these parameters, generic parameter values are obtained from BRENDA and is also processed through PROMENADE.
1. Km for CDP-ME
2. Km for ATP
3. Km for CDP-ME2P
4. Km for ADP
5. Forward Kcat
6. Equilibrium constant (Keq)
References
- ↑ Hanekom