Difference between revisions of "CMK"
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4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinase (CMK) catalyses the production of CDP-ME2P from CDP-ME. This ATP-dependent reaction is the fourth step in the non-mevalonate or methylerythritol-phosphate (MEP) pathway. | 4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinase (CMK) catalyses the production of CDP-ME2P from CDP-ME. This ATP-dependent reaction is the fourth step in the non-mevalonate or methylerythritol-phosphate (MEP) pathway. | ||
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| + | |||
| + | == Equation Rate == | ||
| + | |||
| + | The reversible Michaelis-Menten equation to model the dynamic changes of CMK is: | ||
| + | |||
| + | :<math> | ||
| + | |||
| + | V_\mathrm{CMK} = \cfrac{\left ( Kcat_\mathrm{forward} \cdot [CMK]\right ) \cdot \left (\cfrac{[CDP-ME]}{Km_\mathrm{CDP-ME}} \right ) \cdot \left(\cfrac{[ATP]}{Km_\mathrm{ATP}} \right) \cdot \left (1 - \cfrac{\cfrac{[CDP-ME2P]\cdot[ADP]}{[CDPME]\cdot[ATP]}}{K_\mathrm{eq}} \right)}{\left(1 + \cfrac{[CDP-ME]}{Km_\mathrm{CDP-ME}} + \cfrac{[ADP]}{Km_\mathrm{ADP}}\right)\cdot \left (1+ \cfrac{[ATP]}{Km_\mathrm{ATP}} +\cfrac{[CDP-ME2P]}{Km_\mathrm{CDP-ME2P}} \right)} | ||
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| + | |||
| + | </math> | ||
| + | |||
| + | '''where :''' | ||
| + | |||
| + | |||
| + | {| class="wikitable" | ||
| + | ! scope="col" style="width: 50px; background: #ADD8E6; " | Parameter | ||
| + | ! scope="col" style="width: 225px; background: #ADD8E6;" | Description | ||
| + | ! scope="col" style="width: 50px; background: #ADD8E6;" | Units | ||
| + | |- | ||
| + | | V<sub>CMK</sub> || Reaction rate for CMK || μM/min | ||
| + | |- | ||
| + | | K<sub>cat<sub>forward</sub></sub> || Forward turnover number || min<sup>-1</sup> | ||
| + | |- | ||
| + | | Km<sub>CDPME</sub> || Michaelis-Menten constant for CDPME || μM | ||
| + | |- | ||
| + | | Km<sub>ATP</sub> || Michaelis-Menten constant for ATP || μM | ||
| + | |- | ||
| + | | Km<sub>CDPME2P</sub> || Michaelis-Menten constant for CDPME2P||μM | ||
| + | |- | ||
| + | | Km<sub>ADP</sub> || Michaelis-Menten constant for ADP || μM | ||
| + | |- | ||
| + | | K<sub>eq</sub> || Equilibrium constant || unitless | ||
| + | |- | ||
| + | | [CDPME] || CDPME concentration || μM | ||
| + | |- | ||
| + | | [Limonene] || Limonene concentration || μM | ||
| + | |- | ||
| + | | [PP]|| PP concentration || μM | ||
| + | |} | ||
Revision as of 18:19, 7 March 2017
The reaction
4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinase (CMK) catalyses the production of CDP-ME2P from CDP-ME. This ATP-dependent reaction is the fourth step in the non-mevalonate or methylerythritol-phosphate (MEP) pathway.
Equation Rate
The reversible Michaelis-Menten equation to model the dynamic changes of CMK is:
![V_\mathrm{CMK} = \cfrac{\left ( Kcat_\mathrm{forward} \cdot [CMK]\right ) \cdot \left (\cfrac{[CDP-ME]}{Km_\mathrm{CDP-ME}} \right ) \cdot \left(\cfrac{[ATP]}{Km_\mathrm{ATP}} \right) \cdot \left (1 - \cfrac{\cfrac{[CDP-ME2P]\cdot[ADP]}{[CDPME]\cdot[ATP]}}{K_\mathrm{eq}} \right)}{\left(1 + \cfrac{[CDP-ME]}{Km_\mathrm{CDP-ME}} + \cfrac{[ADP]}{Km_\mathrm{ADP}}\right)\cdot \left (1+ \cfrac{[ATP]}{Km_\mathrm{ATP}} +\cfrac{[CDP-ME2P]}{Km_\mathrm{CDP-ME2P}} \right)}](/wiki/images/math/a/2/1/a21c4c09a7ae86ed7025f85bd7f20408.png)
where :
| Parameter | Description | Units |
|---|---|---|
| VCMK | Reaction rate for CMK | μM/min |
| Kcatforward | Forward turnover number | min-1 |
| KmCDPME | Michaelis-Menten constant for CDPME | μM |
| KmATP | Michaelis-Menten constant for ATP | μM |
| KmCDPME2P | Michaelis-Menten constant for CDPME2P | μM |
| KmADP | Michaelis-Menten constant for ADP | μM |
| Keq | Equilibrium constant | unitless |
| [CDPME] | CDPME concentration | μM |
| [Limonene] | Limonene concentration | μM |
| [PP] | PP concentration | μM |
