Difference between revisions of "ATPase"

Latest revision as of 15:22, 28 October 2014

ATPases catalyze the decomposition of ATP into ADP and a free phosphate ion. ^[1]

ATP \rightarrow ADP + Pi

Mass action rate law is used in which $K$ was the rate constant and ATP was the substrate concentration

v = K \times [ATP]

Parameter	Value	Organism	Remarks
$K$	$3 \times 10^{-3}$ ^[2]	HeLa cell line

Three value of K for ATPase has been reported in Hernandez (2011) et. al. ^[2]: 0.0042, 0.0045 for Normoxia and 0.003 for Hypoxia. Taking the average and calculating the Std. Dev. the value is $0.0039 \pm 0.00079$ . Hernandez (2011) et. al. ^[2] reported these values to be adjusted. As the Vmax values were reported in math>U\cdot(\text{mg protein})^{-1}</math>, therefore we adjust these value by multiplying with 65. This gives the value $0.2535 \pm 0.05$

Parameter	Value	Organism	Remarks
$K$	$0.2535 \pm 0.05$	HeLa cell line

↑ Geider, K. and Hofmann-Berling, H. (1981). Proteins controlling the helical structure of DNA. Annu. Rev. Biochem. 50: 233–260.
↑ ^2.0 ^2.1 ^2.2 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)

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 ==Parameters with uncertainty==
-* Three value of K for ATPase has been reported in Hernandez (2011) et. al. <ref name="Hernandez2011"></ref>: 0.0042, 0.0045 for Normoxia and 0.003 for Hypoxia. Taking the average and calculating the Std. Dev. the value is <math>0.0039 \pm 0.00079</math>
+* Three value of K for ATPase has been reported in Hernandez (2011) et. al. <ref name="Hernandez2011"></ref>: 0.0042, 0.0045 for Normoxia and 0.003 for Hypoxia. Taking the average and calculating the Std. Dev. the value is <math>0.0039 \pm 0.00079</math>. Hernandez (2011) et. al. <ref name="Hernandez2011"></ref> reported these values to be adjusted. As the Vmax values were reported in math>U\cdot(\text{mg protein})^{-1}</math>, therefore we adjust these value by multiplying with 65. This gives the value <math>0.2535 \pm 0.05</math>
 {|class="wikitable"
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 |-
 |<math>K</math>
-|<math>0.0039 \pm 0.00079</math>
+|<math>0.2535 \pm 0.05</math>
 |HeLa cell line
 |