Return to overview
The liberation of free AA from the phospholipid membrane. As this reaction is performed by a multitude of isoforms of PLA2, all with unique kinetics, this reaction was simplified.
Reaction
Chemical equation
Failed to parse (unknown function "\rightharpoon"): \rightharpoon AA
Rate equation
Parameters
The name, shorthand, initial value, adapted value and justification for each event parameter.
Parameter Name |
Parameter Shorthand |
Initial Value |
Justification
|
Doubling time of the AA event |
AADT |
420 min |
AA was set to be released for 6h, as this was the length of the inflammatory window in the modelled system. This value was not changed in the modelled scenarios.
|
Maximum concentration of AA |
c |
0 mM |
The adapted value of this parameter depends on the scenario (Table *). This value changes 60 min into the simulation for all scenarios.
|
Decay switch |
c2 |
0 |
If required the production of AA can decrease over time by amending this value from 0 to 1. This was set to 0 in all scenarios.
|
Half-life of the AA event |
AAHL |
420 min |
This value was arbitrarily set at 420 min. However, no simulations required AA release to decrease over time.
|
In silico experiment AA values
In silico experiment |
HaCaT keratinocyte + A23187 |
46BR.1N fibroblast +A23187 |
HaCaT keratinocyte + Indomethacin and A23187 |
46BR.1N fibroblast + Indomethacin and A23187 |
HaCaT keratinocyte + ATP |
46BR.1N fibroblast + ATP |
HaCaT keratinocyte + UVR |
46BR.1N fibroblast + UVR
|
Σ AA released (pg/million cells) |
2.03 x106 |
5.43 x105 |
1.64 x106 |
2.11 x105 |
8.43 x105 |
3.29 x104 |
1.80 x106 |
4.30 x105
|
Σ AA released (mM) |
5.77 x10-4 |
1.54 x10-4 |
4.65 x10-4 |
6.00 x10-5 |
2.40 x10-4 |
9.36 x10-6 |
5.11 x10-4 |
1.22E x10-4
|