Difference between revisions of "AA Release Event"
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! In silico experiment !! HaCaT keratinocyte + A23187 !! 46BR.1N fibroblast +A23187 !! HaCaT keratinocyte + Indomethacin and A23187 !! 46BR.1N fibroblast + Indomethacin and A23187 !! HaCaT keratinocyte + ATP !! 46BR.1N fibroblast + ATP !! HaCaT keratinocyte + UVR !! 46BR.1N fibroblast + UVR | ! In silico experiment !! HaCaT keratinocyte + A23187 !! 46BR.1N fibroblast +A23187 !! HaCaT keratinocyte + Indomethacin and A23187 !! 46BR.1N fibroblast + Indomethacin and A23187 !! HaCaT keratinocyte + ATP !! 46BR.1N fibroblast + ATP !! HaCaT keratinocyte + UVR !! 46BR.1N fibroblast + UVR | ||
|- | |- | ||
| − | | Σ AA released (pg/million cells) || 2.03 | + | | Σ AA released (pg/million cells) || 2.03 x10<sup>6</sup> || 5.43 x10<sup>5</sup> || 1.64 x10<sup>6</sup> || 2.11 x10<sup>5</sup> || 8.43 x10<sup>5</sup> || 3.29 x10<sup>4</sup> || 1.80 x10<sup>6</sup> || 4.30 x10<sup>5</sup> |
|- | |- | ||
| − | | Σ AA released (mM) || 5.77 x10-4 || 1.54 x10-4 || 4.65 x10-4 || 6.00 x10-5 || 2.40 x10-4 || 9.36 x10-6 || 5.11 x10-4 || 1.22E x10-4 | + | | Σ AA released (mM) || 5.77 x10<sup>-4</sup> || 1.54 x10<sup>-4</sup> || 4.65 x10<sup>-4</sup> || 6.00 x10<sup>-5</sup> || 2.40 x10<sup>-4</sup> || 9.36 x10<sup>-6</sup> || 5.11 x10<sup>-4</sup> || 1.22E x10<sup>-4</sup> |
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Revision as of 09:43, 17 May 2019
The liberation of free AA from the phospholipid membrane. As this reaction is performed by a multitude of isoforms of PLA2, all with unique kinetics, this reaction was simplified.
Reaction
Rate equation
Parameters
| Parameter Name | Parameter Shorthand | Initial Value | Justification |
|---|---|---|---|
| Doubling time of the AA event | AADT | 420 min | AA was set to be released for 6h, as this was the length of the inflammatory window in the modelled system. This value was not changed in the modelled scenarios. |
| Maximum concentration of AA | c | 0 mM | The adapted value of this parameter depends on the scenario (Table *). This value changes 60 min into the simulation for all scenarios. |
| Decay switch | c2 | 0 | If required the production of AA can decrease over time by amending this value from 0 to 1. This was set to 0 in all scenarios. |
| Half-life of the AA event | AAHL | 420 min | This value was arbitrarily set at 420 min. However, no simulations required AA release to decrease over time. |
| In silico experiment | HaCaT keratinocyte + A23187 | 46BR.1N fibroblast +A23187 | HaCaT keratinocyte + Indomethacin and A23187 | 46BR.1N fibroblast + Indomethacin and A23187 | HaCaT keratinocyte + ATP | 46BR.1N fibroblast + ATP | HaCaT keratinocyte + UVR | 46BR.1N fibroblast + UVR |
|---|---|---|---|---|---|---|---|---|
| Σ AA released (pg/million cells) | 2.03 x106 | 5.43 x105 | 1.64 x106 | 2.11 x105 | 8.43 x105 | 3.29 x104 | 1.80 x106 | 4.30 x105 |
| Σ AA released (mM) | 5.77 x10-4 | 1.54 x10-4 | 4.65 x10-4 | 6.00 x10-5 | 2.40 x10-4 | 9.36 x10-6 | 5.11 x10-4 | 1.22E x10-4 |
