46BR.1N fibroblasts Fatty acid analysis Results

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The aim of this study was to estimate the levels of AA in cells which were unstimulated and stimulated with A23187 (with/without COX inhibition), ATP and UVR. GC-FID was used for this fatty acid analysis and the values were normalized using cell numbers (ug/106 cells).

The estimated AA levels in 46BR.1N fibroblasts (unstimulated, A23187 (5µM), COX inhibited and A23187 (5 µM), ATP (2 mM) and UVR (15 mJ/cm2)) for 0.5, 1, 3 and 6h. Analysis was performed using GC-FID. Results are expressed mean ± SD (ug/106 cells) of three independent experiments.

Time (h)	Experiment
	Unstimulated	A23187	Indomethacin + A23187	UVR	ATP
0	1.13 ± 0.25	0.96 ± 0.00	0.64 ± 0.49	0.97 ± 0.46	1.00 ± 0.15
0.5	1.12 ± 0.27	0.44 ± 0.32	0.63 ± 0.15	0.78 ± 0.24	0.98 ± 0.13
1	1.09 ± 0.32	0.34 ± 0.13	0.84 ± 0.59	0.71 ± 0.18	0.98 ± 0.17
3	0.97 ± 0.19	0.51 ± 0.34	0.65 ± 0.13	0.58 ± 0.14	0.96 ± 0.12
6	1.03 ± 0.09	0.42 ± 0.23	0.79 ± 0.09	0.54 ± 0.04	1.03 ± 0.22

Levels of cellular arachidonic acid in 46BR.1N fibroblasts following stimulation with (A) A23187, (B) UVR and (C) ATP 6h post stimulation. Data points are from n=3 independent experiments. Non-linear lines of best fit are shown for untreated control cells (red), treated cells (blue) and treated with indomethacin and A23187 cells (black). Abbreviations: control cells (CTR), calcium ionophore (A23187, 5 µM) (CaI) and indomethacin (10 mM) (IND).