Return to overview
Return to 46BR.1N Fibroblasts
The aim of this study was to estimate the levels of AA in cells which were unstimulated and stimulated with A23187 (with/without COX inhibition), ATP and UVR. GC-FID was used for this fatty acid analysis and the values were normalized using cell numbers (ug/106 cells).
Summary Table
The estimated AA levels in 46BR.1N fibroblasts (unstimulated, A23187 (5µM), COX inhibited and A23187 (5 µM), ATP (2 mM) and UVR (15 mJ/cm2)) for 0.5, 1, 3 and 6h. Analysis was performed using GC-FID. Results are expressed mean ± SD (ug/106 cells) of three independent experiments.
| Time (h)
|
Experiment
|
| Unstimulated
|
A23187
|
Indomethacin + A23187
|
UVR
|
ATP
|
| 0
|
1.13 ± 0.25
|
0.96 ± 0.00
|
0.64 ± 0.49
|
0.97 ± 0.46
|
1.00 ± 0.15
|
| 0.5
|
1.12 ± 0.27
|
0.44 ± 0.32
|
0.63 ± 0.15
|
0.78 ± 0.24
|
0.98 ± 0.13
|
| 1
|
1.09 ± 0.32
|
0.34 ± 0.13
|
0.84 ± 0.59
|
0.71 ± 0.18
|
0.98 ± 0.17
|
| 3
|
0.97 ± 0.19
|
0.51 ± 0.34
|
0.65 ± 0.13
|
0.58 ± 0.14
|
0.96 ± 0.12
|
| 6
|
1.03 ± 0.09
|
0.42 ± 0.23
|
0.79 ± 0.09
|
0.54 ± 0.04
|
1.03 ± 0.22
|
Unstimulated Cells
Estimation of AA in unstimulated 46BR.1N fibroblasts over 6h. The data presented is from three independent experiments.
Calcium Ionophore
Estimation of AA in 46BR.1N fibroblasts stimulated with A23187 (5 µM) for 6h. The data presented is from three independent experiments.
ATP
Estimation of AA in 46BR.1N fibroblasts stimulated with ATP (2 mM) for 6h. The data presented is from three independent experiments.
UVR
Estimation of AA in 46BR.1N fibroblasts over 6h following stimulation with UVR (15 mJ/cm2). The data presented is from three independent experiments.
Calcium Ionophore + COX inhibition
Estimation of AA in 46BR.1N fibroblasts inhibited with indomethacin (10 mM, 1h) and stimulated with A23187 (5 µM) for 6h. The data presented is from three independent experiments.
-1.jpg)
