Difference between revisions of "46BR.1N fibroblasts Fatty acid analysis Results"
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The aim of this study was to estimate the levels of AA in cells which were unstimulated and stimulated with A23187 (with/without COX inhibition), ATP and UVR. GC-FID was used for this fatty acid analysis and the values were normalized using cell numbers (ug/106 cells). | The aim of this study was to estimate the levels of AA in cells which were unstimulated and stimulated with A23187 (with/without COX inhibition), ATP and UVR. GC-FID was used for this fatty acid analysis and the values were normalized using cell numbers (ug/106 cells). | ||
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|+ style="text-align: left;" |The estimated AA levels in 46BR.1N fibroblasts (unstimulated, A23187 (5µM), COX inhibited and A23187 (5 µM), ATP (2 mM) and UVR (15 mJ/cm2)) for 0.5, 1, 3 and 6h. Analysis was performed using GC-FID. Results are expressed mean ± SD (ug/106 cells) of three independent experiments. | |+ style="text-align: left;" |The estimated AA levels in 46BR.1N fibroblasts (unstimulated, A23187 (5µM), COX inhibited and A23187 (5 µM), ATP (2 mM) and UVR (15 mJ/cm2)) for 0.5, 1, 3 and 6h. Analysis was performed using GC-FID. Results are expressed mean ± SD (ug/106 cells) of three independent experiments. | ||
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| − | [[Image: | + | [[Image:Firbo_AA.jpg|none|thumb|500px|Levels of cellular arachidonic acid in 46BR.1N fibroblasts following stimulation with (A) A23187, (B) UVR and (C) ATP 6h post stimulation. Data points are from n=3 independent experiments. Non-linear lines of best fit are shown for untreated control cells (red), treated cells (blue) and treated with indomethacin and A23187 cells (black). Abbreviations: control cells (CTR), calcium ionophore (A23187, 5 µM) (CaI) and indomethacin (10 mM) (IND). ]] |
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Revision as of 14:09, 26 August 2019
The aim of this study was to estimate the levels of AA in cells which were unstimulated and stimulated with A23187 (with/without COX inhibition), ATP and UVR. GC-FID was used for this fatty acid analysis and the values were normalized using cell numbers (ug/106 cells).
| Time (h) | Experiment | ||||
|---|---|---|---|---|---|
| Unstimulated | A23187 | Indomethacin + A23187 | UVR | ATP | |
| 0 | 1.13 ± 0.25 | 0.96 ± 0.00 | 0.64 ± 0.49 | 0.97 ± 0.46 | 1.00 ± 0.15 |
| 0.5 | 1.12 ± 0.27 | 0.44 ± 0.32 | 0.63 ± 0.15 | 0.78 ± 0.24 | 0.98 ± 0.13 |
| 1 | 1.09 ± 0.32 | 0.34 ± 0.13 | 0.84 ± 0.59 | 0.71 ± 0.18 | 0.98 ± 0.17 |
| 3 | 0.97 ± 0.19 | 0.51 ± 0.34 | 0.65 ± 0.13 | 0.58 ± 0.14 | 0.96 ± 0.12 |
| 6 | 1.03 ± 0.09 | 0.42 ± 0.23 | 0.79 ± 0.09 | 0.54 ± 0.04 | 1.03 ± 0.22 |
Levels of cellular arachidonic acid in 46BR.1N fibroblasts following stimulation with (A) A23187, (B) UVR and (C) ATP 6h post stimulation. Data points are from n=3 independent experiments. Non-linear lines of best fit are shown for untreated control cells (red), treated cells (blue) and treated with indomethacin and A23187 cells (black). Abbreviations: control cells (CTR), calcium ionophore (A23187, 5 µM) (CaI) and indomethacin (10 mM) (IND).
