Difference between revisions of "46BR.1N fibroblasts Fatty acid analysis Results"
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+ | [[Welcome to the In-Silico Model of Cutaneous Lipids Wiki | Return to overview]] | ||
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+ | [[46BR.1N Fibroblasts|Return to 46BR.1N Fibroblasts]] | ||
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+ | The aim of this study was to estimate the levels of AA in cells which were unstimulated and stimulated with A23187 (with/without COX inhibition), ATP and UVR. GC-FID was used for this fatty acid analysis and the values were normalized using cell numbers (ug/106 cells). | ||
== Summary Table == | == Summary Table == | ||
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| style="text-align: center;" | 1.03 ± 0.22 | | style="text-align: center;" | 1.03 ± 0.22 | ||
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+ | == Unstimulated Cells == | ||
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+ | [[Image:F06_AA_(ug_million_cells)-1.jpg|none|thumb|500px|Estimation of AA in unstimulated 46BR.1N fibroblasts over 6h. The data presented is from three independent experiments. ]] | ||
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+ | == Calcium Ionophore == | ||
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+ | [[Image:F03-1.jpg|none|thumb|500px| Estimation of AA in 46BR.1N fibroblasts stimulated with A23187 (5 µM) for 6h. The data presented is from three independent experiments. ]] | ||
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+ | == ATP == | ||
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+ | [[Image:F04-1.jpg|none|thumb|500px| Estimation of AA in 46BR.1N fibroblasts stimulated with ATP (2 mM) for 6h. The data presented is from three independent experiments.]] | ||
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+ | == UVR == | ||
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+ | [[Image:F01-1.jpg|none|thumb|500px| Estimation of AA in 46BR.1N fibroblasts over 6h following stimulation with UVR (15 mJ/cm2). The data presented is from three independent experiments.]] |
Revision as of 07:38, 3 June 2019
The aim of this study was to estimate the levels of AA in cells which were unstimulated and stimulated with A23187 (with/without COX inhibition), ATP and UVR. GC-FID was used for this fatty acid analysis and the values were normalized using cell numbers (ug/106 cells).
Summary Table
Time (h) | Experiment | ||||
---|---|---|---|---|---|
Unstimulated | A23187 | Indomethacin + A23187 | UVR | ATP | |
0 | 1.13 ± 0.25 | 0.96 ± 0.00 | 0.64 ± 0.49 | 0.97 ± 0.46 | 1.00 ± 0.15 |
0.5 | 1.12 ± 0.27 | 0.44 ± 0.32 | 0.63 ± 0.15 | 0.78 ± 0.24 | 0.98 ± 0.13 |
1 | 1.09 ± 0.32 | 0.34 ± 0.13 | 0.84 ± 0.59 | 0.71 ± 0.18 | 0.98 ± 0.17 |
3 | 0.97 ± 0.19 | 0.51 ± 0.34 | 0.65 ± 0.13 | 0.58 ± 0.14 | 0.96 ± 0.12 |
6 | 1.03 ± 0.09 | 0.42 ± 0.23 | 0.79 ± 0.09 | 0.54 ± 0.04 | 1.03 ± 0.22 |