46BR.1N Fibroblasts

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The 46BR.1N human fibroblast cell line was purchased from The European Collection of Cell Culture (ECACC) (Salisbury, UK). This cell line represents immortalised dermal fibroblasts, showing normal cell morphology and function (Arlett et al., 1988). The recommended cell culture medium for 46BR.1N fibroblasts was MEME containing 15% FBS, L-glutamine (200nM), non-essential amino acids (200 nM) and sodium pyruvate (200 nM).