3-phosphoglycerate kinase
3-Phosphoglycerate kinase (PGK) is an enzyme that catalyzes the reversible transfer of a phosphate group from 1,3-bisphosphoglycerate (1,3-BPG) to ADP producing 3-phosphoglycerate (3-PG) and ATP. Like all kinases it is a transferase.
Contents
Chemical equation
Rate equation
Random Bi-Bi reversible Michaelis-Menten euation for non-interacting substrates are used. [1]
Modified rate law to consider thermodynamic constraint
Parameter values
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
8.7 [2] | HeLa cell line | |||
2.5[1] | ||||
0.079[1] | mM | |||
0.13[1] | mM | |||
0.04[1] | mM | |||
0.27[1] | mM |
Parameters with uncertainty
- As the value of the does not depend on the organism, the mean and std. dev. of the distribution can be calculated from the various values reported in the literature. [3]
Alternative: Same percent of error reported for can be considered for while the mean value as reported in [2] can be considered. In that case the value would be
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
[2] | Human | |||
Sampled based on Haldane relation or Alternative value | ||||
[4] | mM | |||
[4] | mM | |||
[4] | mM | |||
[4] | mM |
Equilibrium constant
Equilibrium constant | Conditions | Source |
---|---|---|
1974.16 | pH=7, T=25°C | Lehninger, (2008)[5] p 553: , |
3333 | pH=6.9, T=298.15 K | From Krietsch et al. (1970) [6] |
3571 | pH=6.9, T=298.15 K | From Krietsch et al. (1970) [6] |
- Taking average from these values give
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
- ↑ 2.0 2.1 2.2 Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
- ↑ Achcar, F., Kerkhoven, E. J., Bakker, B. M., Barrett, M. P., Breitling, R. (2012), Dynamic modelling under uncertainty: the case of Trypanosoma brucei energy metabolism, PLoS Comput. Biol. 8, e1002352.
- ↑ 4.0 4.1 4.2 4.3 Szabo, J.; Varga, A.; Flachner, B.; Konarev, P.V.; Svergun, D.I.; Zavodszky, P.; Vas, M. (2008), Role of side-chains in the operation of the main molecular hinge of 3-phosphoglycerate kinase, FEBS Lett. 582, 1335-1340
- ↑ David L. Nelson, Michael M. Cox (2008), Lehninger Principles of Biochemistry (5th edn), W. H. Freeman and Company
- ↑ 6.0 6.1 Krietsch WZ and Bücher T. (1970) Eur J Biochem. 17(3):568-80. [pmid: 5493986]