HaCaT Fatty acid analysis Results
The aim of this study was to estimate the levels of AA in cells which were unstimulated and stimulated with A23187 (with/without COX inhibition), ATP and UVR. GC-FID was used for this fatty acid analysis and the values were normalized using cell numbers (ug /106 cells).
Time (h) | Experiment | ||||
---|---|---|---|---|---|
Unstimulated | A23187 | Indomethacin + A23187 | UVR | ATP | |
0 | 2.87 ± 0.28 | 2.87 ± 0.28 | 2.87 ± 0.28 | 2.87 ± 0.28 | 2.87 ± 0.28 |
0.5 | 2.68 ± 0.49 | 0.28 ± 0.07 | 4.18 ± 0.47 | 2.41 ± 0.70 | 1.27 ± 0.23 |
1 | 2.57 ± 1.25 | 0.21 ± 0.09 | 3.42 ± 0.47 | 2.15 ± 0.72 | 1.44 ± 0.41 |
3 | 2.51 ± 0.82 | 0.25 ± 0.20 | 1.98 ± 0.34 | 1.74 ± 0.77 | 1.15 ± 0.20 |
6 | 2.91 ± 0.22 | 0.28 ± 0.35 | 1.13 ± 0.49 | 0.79 ± 0.82 | 1.47 ± 0.28 |