Transformation of AA to 5-HPETE

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5-LOX activity in the skin remains a mystery. The enzyme has a low levels of activity in cutaneous cells, but products of the pathway such as leukotrienes and 5-HETE are always detected in human skin tissue (Breton, Woolf et al. 1996). As with various prostaglandins found in the compartment, it has been suggested that the detected 5-LOX metabolites are as a result of infiltrating cells from the vascular compartment (Kowal-Bielecka, Distler et al. 2001, Kanaoka and Boyce 2004).


Reaction

R11 AA - HPETE5.jpg

Chemical equation

 PGH2 \rightleftharpoons 5-HPETE

Rate equation

R11.PNG

5-LOX Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
5.10E-03  mM Human Expression Vector: Baculovirus, Sf9 insect cells

Enzyme: Recombinant 5-Lipoxygenase pH: 5.6 Temperature:37

[1]
1.20E-02  mM Human Expression Vector: Polymorphonuclear Leukocytes

Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22

[2]
6.31E-02  mM Human Expression Vector: Polymorphonuclear Leukocytes

Enzyme: 5-Lipoxygenase pH:7.5 Temperature: 22

[3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
1500 + 75 per minute Potato Expression Vector:Potato Tubers

Enzyme: 5-Lipoxygenase pH:5.5 Temperature: 23

[4]


5-LOX Abundance
Value Units Species Notes Reference
97.3  ppm Human Expression Vector: Lung

Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C

[5]
49.8  ppm Human Expression Vector: Esophagus

Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C

[6]
31.9  ppm Human Expression Vector: Oral Cavity

Enzyme: 5-LOX pH: 7.5 Temperature: 37 °C

[6]
Gibbs Free Energy Change
Value Units Species Notes Reference
(-15.9) - 18.01 kcal/mol Soybean Expression Vector: Soybean

Enzyme: Lipoxygenase-1 pH:Not stated Temperature: Not stated

[7]
(-69.979996) kcal/mol Not stated Estimated

Enzyme: 5-Lipoxygenase Substrate: Arachidonate Product: 5-HPETE pH: 7.3 ionic strength: 0.25

[8]

References


Related Reactions