Lactate dehydrogenase
A dehydrogenase is an enzyme that transfers a hydride from one molecule to another. Lactate dehydrogenase catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD+ and back.
Chemical reactions
![NADH + PYR \rightleftharpoons Lactate_{in} + NAD](/wiki/images/math/2/a/d/2ad51186abb6783280b6c93679dfb9b2.png)
Rate equation
![\frac{V_{mf}\frac{[NADH][PYR]}{K_{NADH} K_{PYR}} - V_{mr}\frac{[Lactate_{in}][NAD]}{K_{Lactate_{in}} K_{NAD}}}{1 + \frac{[NADH]}{K_{NADH}} + \frac{[PYR]}{K_{PYR}} + \frac{[NADH][PYR]}{K_{NADH} K_{PYR}} + \frac{[Lactate_{in}][NAD]}{K_{Lactate_{in}} K_{NAD}} + \frac{[Lactate_{in}]}{K_{Lactate_{in}}} + \frac{[PYR]}{K_{PYR}} }](/wiki/images/math/e/b/0/eb0bc9b0022002ad69cab019a0bd568a.png)
Prameter values
Parameter | Value | Organism | Remarks |
---|---|---|---|
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11.4 [1] | HeLa cell line | |
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1.8[1] | Rat AS-30D hepatoma | |
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0.1[1] | HeLa cell line | |
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4.7[1] | Rat AS-30D hepatoma | |
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0.07[1] | HeLa cell line | |
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0.002[1] | HeLa cell line |