Fructose 1,6 bisphosphate aldolase

This enzyme splits fructose 1, 6-bisphosphate into two sugars that are isomers of each other. These two sugars are Dihydroxyacetone phosphate (DHAP) and Glyceraldehyde 3-phosphate (Gly3P).

Chemical equation

Fru1,6BP \rightleftharpoons Gly3P + DHAP

Reversible Uni-Bi Michaelis-Menten is used. ^[1]

\frac{V_{mf} \frac{[Fru1,6BP]}{K_{Fru1,6BP}} - V_{mr}\frac{[DHAP][G3P]}{K_{DHAP}K_{Gly3P}} }{1 + \frac{[Fru1,6BP]}{K_{Fru1,6BP}} + \frac{[DHAP]}{K_{DHAP}} +\frac{[Gly3P]}{K_{Gly3P}} + \frac{[DHAP][Gly3P]}{K_{DHAP}K_{Gly3P}} }

Parameter	Value	Organism
$V_{mf}$	0.2 ^[2]	Hela cell line
$V_{mr}$	0.18^[2]	Rodent AS-30D hepatoma
$Km_{Fru1,6BP}$	0.009^[2]	Hela cell line
$Km_{Gly3P}$	0.16^[2]	Rodent AS-30D hepatoma
$Km_{DHAP}$	0.08^[2]	Rodent AS-30D hepatoma

↑ Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011) Modeling cancer glycolysis. Biochim Biophys Acta 1807:755–767 (doi)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)