Transformation of AA to 12-HPETE

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Revision as of 14:34, 18 August 2016 by Muttley93 (talk | contribs) (12-LOX Parameters)
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R179AA - HPETE12.jpg

Chemical equation

 AA \rightleftharpoons PGH2

Rate equation

12-LOX Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
7.20E-03  mM Human Expression Vector: Platelet

Enzyme: 12-Lipoxygenase pH: 7.4 Temperature: 37°C.

[1]
8.00E-02  mM Human Expression Vector: Platelet

Enzyme: 12-Lipoxygenase pH: 7 Temperature: 24

[2]
1.00E-02  mM Human Platlet Expression Vector: Baculovirus

Enzyme: 12-Lipoxygenase pH: 8 Temperature: 37

[3]
7.90E-03 ± 8.00E-04  mM Human Expression Vector: Platelets

Enzyme:12-Lipoxygenase pH: 7.4 Temperature: 37

[4]
Enzyme Turnover Numbers
Value Units Species Notes Reference
336 ± 12 per minute Human Expression Vector: Human reticulocyte

Enzyme: 15-lipoxygenase-1 pH: 7.5 Temperature: 25

[5]
504 per minute Wild Boar Expression Vector: E. coli.

Enzyme: 12-Lipoxygenase pH: 7.4 Temperature: 37

[6]


12-LOX Abundance
Value Units Species Notes Reference
19.8  ppm Human Expression Vector: Spleen

Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C

[7]
1.60  ppm Human Expression Vector: Liver

Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C

[8]
0.28  ppm Human Expression Vector: Gut

Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C

[8]
0.11  ppm Human Expression Vector: Pancreas

Enzyme: 12-LOX pH: 7.5 Temperature: 37 °C

[8]
Gibbs Free Energy Change
Value Units Species Notes Reference
(-69.979996) kcal/mol Not stated Estimated

Enzyme: 12-LOX Substrate: Arachidonate Product: 12-HPETE pH: 7.3 ionic strength: 0.25

[9]

References

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