Transformation of AA to 12-HPETE

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Reaction

R179AA - HPETE12.jpg

Chemical equation

 AA \rightleftharpoons PGH2

Rate equation

12-LOX Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
7.20E-03  mM Human Platelet [1]
8.00E-02  mM Human Platelet [2]
1.00E-02  mM Human Recombinant Histidine-Tagged Human Platelet 12-Lipoxygenase [3]
7.90E-03 ± 8.00E-04  mM Human Platelet 12-Lipoxygenase [4]
Enzyme Turnover Numbers
Value Units Species Notes Reference
336 ± 12 per minute Human Human reticulocyte 15-lipoxygenase-1, Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5,

25C), with and without product (12-HETE) addition, in the presence of 25 μM of substrate.

[5]
504 per minute Wild Boar Wild Type Leukocyte-Type 12-Lipoxygenase Expressed in E. coli. [6]
Enzyme Concentrations
Value Units Species Notes Reference
5.00e-06 - 6.00e-05 mM Human Human reticulocyte 15-lipoxygenase-1, Enzymatic assays were performed in 25 mM Hepes buffer (pH 7.5,

25C), with and without product (12-HETE) addition, in the presence of 25 μM of substrate.

[5]

References

Related Reactions